摘要
目的探讨优化的噻唑蓝(MTT)比色分析法在预测肝癌天然耐药中的作用。方法改良的 MTT 比色分析法检测30例未经化疗肝癌标本的药物敏感性,相应癌组织石蜡标本构建组织芯片。免疫组化检测 P-gp、MRP3、LRP、GST-π、p16^(INK4a)和 p21^(WAF1)的表达。结果 30例肝癌中耐药者16例(53.3%),敏感者14例(46.7%)。P-gp 在耐药组癌组织中的强阳性表达率(56.3%)明显高于敏感组(14.3%,P<0.05)。结论优化的 MTT 方法更为科学。肝癌普遍存在天然耐药性,P-gp 可以作为肝癌天然耐药的预测指标。
Objective To investigate the role of the improved MTT assay in prediction of intrinsic drug resistance of liver cancer. Methods The convenient MTT eolorimetry was innovated to test the effects of 4＇-epi-adriamyein （E-ADM）, earboplatin （CBP）, and 5-fluoraeil （5-Fu）, used alone or in combination, on 30 specimens of primary liver cancer without chemotherapy. All of the 30 paraffinembedded tissues were assembled in a microarray. The used terminal concentrations of drugs were one twentieth those of the plasma peak concentrations calculated by using the liver cancer cells of the line SMMC- 7721. The expression of P-glycoprotein （P-gp）, multidrug resistant protein （MRP）-3, lung resistancerelated protein （LRP） , glutathione S-transferase （GST）-π, and 2 kinds of cyclin-related protein： p16^INK4a and p21WAF1, were detected by immunohistochemistry. Results Sixteen of the 30 specimens （53.3%） were drug-resistant and 14 of the 30 specimens （46.7%） were drug-sensitive. The sequence of drug - sensitivity was in the order of combination chemotherapy, E-ADM, 5_Fu, and CBP. The positive rate of P- gp in the drug-resistant group was 56. 3%, significantly higher than that of the drug-sensitive group （ 14. 3%, P 〈 0.05）. Conclusion An improved MTT assay has been developed that is more scientific and worth spreading clinically. Intrinsic drug resistance of liver cancer is popular. P-gp is a good predictive marker in intrinsic drug resistance of liver cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第5期333-335,共3页
National Medical Journal of China
关键词
肝肿瘤
比色法
抗药性
肿瘤
Liver neoplasms
Colorimetry
Drug resistance, neoplasm
