摘要
目的确定维生素K3(VK3)在MTT比色法检测活菌数量时的实验用量。方法微孔板和MTT比色法检测不同VK3含量在测量活菌数量中的作用。结果OD值随着菌浓度、VK3含量的升高而增大。溶解剂作用时间对实验组影响不大。菌浓度高时测定波长对OD值有显著影响,反之则影响不显著。结论菌浓度在106数量级,VK3含量为0.1mmol·L-1,测定波长为570nm时所得OD值结果较为可靠、稳定。
AIM： vitamin K3 （ VK3 ） in To determine the concentration of fungal counting with MTT assay METHODS： Microplate and MTT colorimetric assay were used to measure the effect of different VK3 concentrations on fungal counting. RESULTS ： The OD （ optical density） value was affected by fungal measure wavelengh, and solvent and VK3 concentration, addition-time at the same time. Generally speaking, the OD value was increased with fungal and VK3 concentration increased. At a higher fungal concentration, there was little impact from solvent addition-time and remarkably impact from measure wavelengh. CONCLUSION： The reliable and stable results will be acquired in the conditions as follows： 106 fungal concentration, 0.1 mol·L^-4 VK3 concentration, and 570 nm measure wavelengh.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第12期1372-1375,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
