摘要
目的尝试建立鼻咽癌(NPC)化疗药敏药物的体外筛选方法。方法利用四甲基偶氮唑蓝(MTT)法体外药敏试验检测48例NPC对7种临床化疗药物的敏感性,并且与临床化疗效果进行对比。结果NPC细胞对DDP、5-Fu、VCR、CTX、BLM、HHA、VM-26的敏感性存在明显个体差异,其敏感率分别为52%、56%、61%、48%、48%、35%、74%。与临床化疗结果对比结果显示:阳性符合率89.4%,阴性符合率80.0%;敏感性94.4%,特异性66.7%。结论MTT可应用于NPC临床筛选药物。
Objective To evaluate the value of screening assay for chemotherapy of Nasopharyngeal carcinoma（NPC） in vitro. Methods The chemosensitivity of 48 NPC samples were detected by means of MTT in vitro and compared with the clinical effect. Results There are significant individual difference in chemosensitivity of NPC cell for DDP（52 % ）, 5 - Fu （ 56 % ）, VCR （ 61% ）, CTX （48 % ）, BLM （ 48 % ）, HHA （ 35 % ）, VM - 26 （ 74 % ）. Positive detective coincidence rate, negative coincidence rate, sensibitity and specificity was 89.4 %, 80.0 %,94.4 % and 66.7 % respectively compared with the clinical effect. Conclusion It may be applied for clinical screening assay for chemotherapy of NPC in vitro by means of MTT.
出处
《浙江临床医学》
2006年第1期19-20,共2页
Zhejiang Clinical Medical Journal
基金
浙江省教委资助(20030785)
