摘要
丛枝菌根 （AM） 真菌的侵染势（Colonization potential ,CP ）和接种势（inoculum potential ,IP ）是菌根学领域非常重要的两个概念.IP已定义为接种物中有活力的真菌繁殖体及结构的数量（Liu ＆ Luo,1994）.而CP的定量描述和测定方法尚未建立.本文将CP 定义为单位数量接种物在侵染初期侵染植物根系的能力,其定量测定公式为：CP=N×L/IP ×T ,其中 N 为单位根长侵入点数＋根内和根外菌丝数＋含有丛枝的细胞数＋泡囊数;L 为每株寄主植物根系总长度;IP 为接种物的接种势单位数;T 为接种后的天数.用棉花 （Gossypium hirsutum）、大豆 （Glycine max）、红三叶（Trifolium pratense）和玉米（Zea mays ）和3种AM真菌Gigaspora margarita （Gim）, Glomus intraradices （Gi）, and Glomus versiforme（Gv）不同剂量（100, 300, 900, 2 700 and 8 100接种势单位）的接种物进行试验,以定量测定CP、以及CP和IP之间的关系.结果表明,在相同数量的IP条件下,不同AM真菌具有不同的CP,应用该研究建立的定量测定方法获得了CP与IP显著相关（p＝0.01,r＝ 0.9161～ 0.9393）的试验结果.CP可以作为评价接种物有效性和质量的指标.它说明了AM真菌侵染初始阶段的侵染能力,因此CP的测定应该在侵染率达到最高之前进行,对3种一年生植物进行的试验表明,最佳测定时间应在接种后4到6周.
Colonization potential （CP） and inoculum potential （IP） of arbuscular mycorrhizal （AM） fungi are very important concepts in mycorrhizology. IP was defined previously as the number of viable fungal propagules and structures in an inoculum, which could be quantitatively determined. However, the quantitative definition and determination of CP remained yet to be developed. In this paper a quantitative concept of CP has been proposed as the colonization capacity in the initial colonizing stage per unit amount of inocula. A formula to determine the CP is proposed as： CP=N×L/IP ×T ,where N = total numbers of entry points ＋ hyphae both in and on roots ＋ cells with arbuscules ＋ vesicles per unit root length, L = total root length per plant, IP = numbers of IP of inocula used, T = days after inoculation. One experiment was carried out with cotton （Gossypium hirsutum cultivar 86-1）, soybean （Glycine max cultivar Ludou 11） and corn （Zea mays cultivar Yedan 13） inoculated with 3 AM fungi, Gigaspora margarita （Gim）, Glomus intraradices （Gi）, and Glomus versiforme（Gv） to determine CP and another experiment was set up with red clover（Trifolium pratense） inoculated with 100, 300, 900, 2 700 and 8 100 IP of Gim, Gi, or Gv per pots （15 × 20 cm） filled with sterilized sand to test the relationship between IP and CP, and to test if CP can be used as a quality indicator. Results showed that different AM fungi had different CP when inoculated at the same IP level. A significant （at 1% level） correlation between CP and IP （r = 0.9161～ 0.9393） was found with the proposed quantitative method in this study. This further suggested that the previously described IP （Liu and Luo,1994） was accurate and IP was a useful parameter for the amount of inoculum, while CP could be used as an indicator of effectiveness of inocula and could reflect the quality of inocula of various AM fungi. Because the CP is defined as the colonization capacity in the initial colonization stage, the CP
出处
《菌物学报》
CAS
CSCD
北大核心
2006年第3期408-415,共8页
Mycosystema
基金
Supported by the National Natural Science Foundation of China (No. 30471164) and Natural Science Foundation of Shandong Province(Z2005D03).
