摘要
为了探索AM真菌(AM真菌)在牡丹种苗生产和观赏栽培中的合理应用,更好地利用其优势AM真菌菌种资源,培育优质牡丹菌根化容器苗,笔者以牡丹(Paeonia suffruticosa)容器苗为接种对象,设计筛选AM真菌接种剂、接种期和栽培基质的最佳组合。结果表明:AM真菌接种剂对牡丹丛枝菌根形成均有显著影响,以Glomus geosporum(Gg)和Glomus mosseae(Gm)混合接种剂在第3剂量水平下效果最显著;随着株龄增大,丛枝菌根侵染效果显著降低,当年生苗侵染率、侵染强度、丛枝着生率、侵入位点数与菌丝长度均最高,分别为94.64%、13.2、51.8%、6.3个/(mm根长)、9.58m(/g.干土),均显著地(P<0.05)高于1年生苗、2年生苗和3年生苗;在备选的4种栽培基质中,以泥炭:珍珠岩:河沙(1:1:1)基质最合适,在接种后112天时侵染率、侵染强度、菌丝长度最高,分别为90.20%、22.50和2.2m/(g.干土),极显著地(P<0.01)高于其他基质。因此,以当年生牡丹容器苗接种Gg+Gm,并采用泥炭:珍珠岩:河沙(1:1:1)的栽培基质最佳。
In order to make use of dominant species of AMF on seedling production and ornamental culture of tree peony,and cultivate high quality container seedlings and potted flowers,the effects of species and dose of AMF,culture substrates,and age of plant on colonization of arbuscular mycorrhizal for tree peony container seedling were investigated.The colonization frequency and intensity ofGlomus geosporum（Gg）mixedGlomus mosseae（Gm）at level 3 held the highest among all treatments.Colonization frequency,colonization intensity,abusculars rate,entry points and hyphal length of annual seedlings held the highest with a significant difference（P 0.05）than others,which were 94.64%,13.2,51.8%,6.3 point/（mm root length）and 9.58 m/（g dry soil）.They decreased with the age of plants.In the 4 cultivating substances,there was a significant difference（P 0.01）among turf：perlite：sand（1：1：1）with other media among colonization frequency（90.2%）,colonization intensity（22.50）and 12.2 m/（g dry soil）.So,it was optimal that annual seedlings inoculated with Gg＋Gm were cultivated in substrates for turf：perlite：sand（1：1：1）.
出处
《中国农学通报》
CSCD
北大核心
2011年第10期108-112,共5页
Chinese Agricultural Science Bulletin
基金
"863"项目牡丹产业化技术研究子课题
国家林业局重点研究项目"牡丹新优品种繁育及区域化试验"(2008-10)
关键词
牡丹
AM真菌
容器苗
丛枝菌根
栽培基质
侵染
Paeonia suffruticosa
AM fungus（AMF）
container seedling
arbuscular mycorrhizal
culture substrates
colonization
