摘要
The effects of inoculum forms (single-spore, multi-spores, or colonized root pieces) and host plants (Nicotiana tabacum L., Sorghum sudanense (Piper) Stapf, and Trifolium repens L.) on the development and inoculum potential (IP) of the arbuscular mycorrhizal fungi (AMF) :Glo-mus macrocarpum Tul & Tul, donuis mosseae (Nicol & Gerd.) Gerdemann & Trappe, Glomus ver-siforme (Karsten) Berch, and Sclerocystis sinu/osa Gerdemann & Bakhi cultured in pots were investigated. The lag phase of treatment with 50 spores or 0.5 g (fresh weight) of colonized root pieces was 4 weeks, much shorter than that of the treatment with 1 spore (8 weeks); the value of IP (VIP) and percentage of root colonization (PRC) of the former were greater than those of the latter. Only on the early stages of colonization was there difference between the 50 spores and the 0.5 g (fresh weight) of colonized root piece inoculation treatments. The EP per plant inoculated with 0.5 g (fresh weight) of colonized root pieces of AMF was greater than that of the other two treatments except G. versiforme on Nicotiana tabacum, while the PRC of the plants inoculated with 50 spores and 0.5 g (fresh weight) of colonized root pieces of AMF was higher than that of the 1 spore inoculation after 10 weeks. Trie VIP of AMF on Trifolium repens was significantly higher than that on the other two hosts. The VIP of G. mosseae, G. versiforme, and S. sinuosa was respectively greater than that of G. macrocarpum. This suggested that different species of AMF produced different VIP of the inoculum . Nicotiana tabacum was much better than the other host plants which used to be inoculated with single spore, and to produce inocula of AMF.
在盆栽条件下研究了丛枝菌根菌(arbuscular mycorrhizal fungi,AMF)单孢、多孢和菌根根段接种物及其寄主植物烟草(Nicotiana tabacum L.)、苏丹草(Sorghum sudanense(Piper)Stapf)和三叶草(Trifoliumrepens L.)对AMF Glomus macrocarpum Tul ＆ Tul、Glomus mosseae(Nicol ＆ Gerd.)Gerdemann ＆ Trappe、Glomusversiforme(Karsten)Berch和Sclerocystis sinuosa Gerdemann ＆ Bakhi发育和接种势值(value of inoculum poten-tial,VIP)的影响。用50个孢子或0.5g菌根根段接种物处理的感染迟缓期为28d,大大短于单孢接种处理(64d);前者的VIP和根系感染百分率(percentage of root colonization,PRC)均高于后者。50个孢子和0.5g菌根根段接种物两处理之间仅在感染的早期阶段存在差异。0.5g菌根根段接种物(烟草上G.versiforme菌除外)接种处理的VIP大于其他两种接种物的处理;而50个孢子或0.5g菌根根段接种物处理的PRC直至接种后70d仍高于单孢接种处理。三叶草上AMF的VIP显著大于其他两种寄主植物上的VIP。G.mosseae、G.versiforme和S.sinuosa的VIP则大于G.macrocarpum的处理。这表明不同AMF菌种能产生不同接种势值的接种物。烟草则是用于单孢接种和接种物生产的良好寄主植物。
基金
Supported by International foundation for Science (IFS)
National Natural Science Foundation of China.
