摘要
通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对药用植物滇重楼根系AM真菌侵染能力和入药质量的影响。结果显示,与对照组(CK)相比,接种外源AM真菌对滇重楼根系AM真菌的孢子密度、侵染率、侵染强度具有调控(增减)作用,增强根内菌丝的琥珀酸脱氢酶(SDH)和碱性磷酸酶(ALP)活性,从而提高滇重楼菌根生活力。AM真菌诱导子处理后,不同AM真菌对滇重楼根茎中不同甾体皂苷的影响有所差异,AM真菌的施用能够提高滇重楼的药用品质,但在实验期间未造成滇重楼甾体皂苷质量的变异。实验结果表明,滇重楼与AM真菌之间的共生关系具有一定的相互选择性,近明球囊霉Claroideoglomus claroideum和瑚状盾巨囊霉Racocetra coralloidea是最适宜滇重楼大田栽培时推广应用的优良接种菌剂。
After 28 foreign species of AM fungi were inoculated in sterilized soil, the effects of the AM mycorrhizal colonization and the medicine quality of Paris polyphylla var. yunnanensis were observed by combination of inoculation test in pot at room temperature and instrumental analysis. The results showed that, compared with control group （CK）, the inoculation of foreign AM fungi in the soil influenced the spore density, mycorrhizal infection rate, and colonization intensity of AM fungi in root system of P. poITphylla var. yunnanensis. The inoculation of foreign AM fungi enhanced the mycorrhiza viability of P. polyphylla var. yunnanensis by increasing the activity of succinic dehydrogenase （SDH） and alkaline phosphatase （ALP） in intraradical hyphae. The content of single steroid sapo- nin in rhizome of P. polyphylla var. yunnanensis showed variation after P. polyphylla var. yunnanensis was inoculated by different for- eign species of AM fungi, which was beneficial for increasing the medicine quality; however, the kinds of steroid saponin showed no difference. In a degree, there was a selectivity of symbiosis between P. polyphylla vat. yunnanensis and foreign AM fungi. And we found that the Claroideoglomus claroideum and Racocetra coralloidea were best foreign AM fungi species for cultivating P. polyphylla var. yunnanensis under field condition.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第16期3158-3167,共10页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81260622)
云南省应用基础研究计划项目(2011FB081)
云南省教育厅科学研究基金重点项目(2012Z119)
关键词
滇重楼
AM真菌
侵染力
琥珀酸脱氢酶
碱性磷酸酶
甾体皂苷
指纹图谱
Paris polyphylla var. yunnanensis
AM fungi
colonization
succinic dehydrogenase
alkaline phosphatase
steroidsaponin
fingerprint
