摘要
以大豆品种黑农48为材料,通过盆栽试验研究了不同硫素水平对结荚期大豆AM真菌侵染率的影响及其菌群结构的变化。结果表明:不同硫水平对大豆AM真菌的侵染率和菌群结构均有影响,其中大豆根系和土壤中AM真菌的多样性在硫素水平为0.02 g.kg-1时较高,当继续增加硫素水平时反而有所降低,不施硫时最低,表明适当施加硫素能够提高AM真菌的多样性,高硫水平反而抑制AM真菌的多样性。通过对DGGE图谱中G1等12条条带的序列分析表明,12条条带对应的菌种均为球囊霉属(Glomus)AM真菌,表明该试验中球囊霉属是大豆根系和根际土壤中的优势AM真菌。
A pot experiment was carried out to research the effect of different sulfur levels on microbial structure of AM fungi and its infection rate of Heinong 48 soybean at podding stage.The results showed that sulfur levels had influences on both microbial structure of AM fungi and its infection rate.When the soil sulfur level was 0.02 g·kg^-1,both the soybean roots and AM fungi had a high diversity.However,when the sulfur level rised,they were both decreased.And the diversity was lowest when no sulfur was added to the soil.These results indicate that addition of sulfur appropriatelycould increase the diversity of AM fungi and when the sulfur level was too high the diversity of AM fungi was decreased.Moreover,the results from analyzing the twelve bands in DGGE map suggest that the twelve bands with the corresponding species are Glomus AM fungi,and the Glomus were the dominant AM fungi both in soybean roots and rhizosphere.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第6期990-995,共6页
Soybean Science
基金
黑龙江省自然科学基金资助项目(C200918)
黑龙江省博士后科研启动基金项目(LBH-Q09022)
关键词
大豆
硫素水平
AM真菌
多样性
DGGE
Soybean
Sulfur
Arbuscular mycorrhizal fungi
Diversity
Denaturing gradient gel electrophoresis
