摘要
目的研究我国9个省28个民族群体Y染色体DYS287位点的遗传多态性。方法应用Touchdown PCR技术扩增1006个DNA样品的Y染色体Alu序列多态性(YAP)+片断,琼脂糖凝胶电泳分离检测。结果藏族YAP+频率为36·7%,土族23·8%,彝族18·4%,普米族11·3%,塔吉克族7·4%,白族6·7%,基诺族5·1%,山东汉族4%,仫佬族2·7%,毛南族1·3%,甘肃汉族、云南汉族、壮族、傣族、黎族、怒族、傈僳族、纳西族、拉祜族、独龙族、哈尼族、畲族、维吾尔族、撒拉族、柯尔克孜族、东乡族、佤族及朝鲜族的YAP+频率均为0。结论YAP+频率在部分汉藏语系民族(藏、彝、普米、基诺、白族)中频率较高。在历史学、语言学上被认为来源于中亚的民族(土族、塔吉克族)中YAP+频率也较高。而起源于百越民族的少数民族(仫佬族、毛南族)YAP+频率值得进一步探讨。
Objective To investigate the polymorphism of DYS287 among 28 ethnic populations in 9 provinces of China. Method YAP element was detected by Touchdown PCR amplification and 2% agarose gel electrophoresis. Results YAP ＋ frequencies in these ethnic populations were as follows： Zang 36.7% , Tu 23.8% , Yi 18.4% , Pumi 11.3% , Tajik 7.4% , Bai 6.7% , Jino 5.1% , Shandong Han 4% , Mulao 2.7% , and Maonan 1.3%. The rest ethnic populations in our study, including Gansu Han, Yunnan Han, Zhuangzu, Daizu, Lizu, Nuzu, Lisu, Naxi, Lahu, Dulong, Hani, Shezu, Weiwuer, Sala, Kerkizi, Dongxiang, Vazu, and Korea didn＇t carry YAP ＋ element. Conclusions Zangzu, Tuzu, Yizu, Pumi, Jino, and Baizu, which belong to Sino-Tibetan language family, carry a high YAP ＋ frequency. Sala, Tuzu, and Ta- jik, regarded as Central Asia by origin in history and linguistics, also have a high YAP ＋ frequency. Mulao and Maonan, which origin from ＂Baiyue＂ ancient ethnic groups, also have a considerable YAP ＋ frequency.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2006年第2期196-201,共6页
Acta Academiae Medicinae Sinicae
基金
美国中华医学基金会基金资助项目(04-805)
国家高技术研究发展计划项目(863项目)(2002AA231051
2002BA711A08)
高等学校优秀青年教师教学科研奖励计划~~
