摘要
目的 :研究我国广西地区不同少数民族及其亚群的遗传学关系。方法 :选择 6个具有遗传稳定性的STR位点 (D3S135 8,vWA ,FGA ,D5S818,D13S317,D7S82 0 )作为分子遗传标记 ,收集中国广西阳朔壮族、宛田瑶族血液样本 ,使用ABI377全自动测序仪进行STR分型 ,并结合已公开发表的广西地区其他民族的 6个STR位点群体遗传学资料 ,进行Hardy Weinberg平衡检验 ,计算H ,PIC ,DP和PPE ,采用Nei′s法计算各人群间的遗传距离 ,采用组间平均连锁法进行聚类分析 ,采用UPGMA法绘制系统发生树。结果 :15个人群的 6个STR位点基因型分布符合Hardy Weinberg平衡定律 (P >0 .0 5 ) ;H分布在 0 .6 4 82～ 0 .92 99,PIC分布在 0 .5 70 6～ 0 .86 6 2 ,DP分布在 0 .82 5 8～ 0 .970 6 ,PPE分布在 0 .4 2 15～ 0 .9917;广西京族与东兴京族的遗传距离最小 (0 .0 0 0 7) ,广西仡佬族与宛田瑶族遗传距离最大 (0 .2 391) ;聚类分析与系统发生树结果一致 ,15个人群大致分为 2组。结论 :6个STR位点在广西15个民族群体中总体上存在较高的杂合度、遗传多态性和个体识别力 ;广西不同民族之间存在一定的基因交流 。
Objective To determine the molecular genetic relationship of minorities and sub-population in Guangxi area. Methods Six stable STRs (D3S1358,vWA,FGA,D5S818,D13S317 and D7S820) were selected as genetic markers. STR data of Zhuang (Yangshuo) and Yao(Wantian) were genotyped from blood samples by ABI377 sequencer. STR data of another 12 ethnic groups in Guangxi were collected from publications. Hardy-Weinberg equilibrium was evaluated and H,PIC, DP and PPE were computed. The genetic distances between different populations were calculated using Nei′s method and clustering analysis was done by average linkage between the groups. We drew their phylogenetic tree using UPGMA. Results The alleles of 6 STRs in the 15 populations of Guangxi conformed with Hardy-Weinberg equilibrium. H was distributed in 0.6482～0.9299; PIC in 0.5706～0.8662;DP in 0.8258～0.9706, and PPE in 0.4215～0.9917. Genetic distance between Jing of Guangxi and Jing in Dongxing was minimum (0.0007) in the 15 populations, while genetic distance between Guangxi Gelao and Wantian Yao was maximal (0.2391). The phylogenetic tree was similar to the results of clustering analysis and all the 15 populations were clustered into 2 groups.Conclusion The results indicate that the H, PIC, DP and PPE of 6 STRs in the 15 Guangxi populations are comparatively high. There is gene flow in some groups, but several minorities are relatively separated.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2004年第6期619-622,共4页
Journal of Central South University ：Medical Science
基金
国家自然科学基金 (39970 4 0 1 )
国家"95"攻关项目 (96 91 9 0 1 0 4 )
