摘要
目的:探讨逍遥散对慢性心理应激大鼠海马突触体内Ca2+浓度及蛋白激酶C(PKC)活性的影响,进一步研究其对心理应激损伤学习记忆保护作用的机制。方法:Wistar大鼠随机分为五组,采用慢性多相性应激模型,以Fura-2负载及荧光分光光度计检测大鼠海马突触体内Ca2+浓度,采用琼脂糖凝胶电泳法检测突触体内PKC活性。结果:两个模型组大鼠海马突触体内Ca2+浓度及PKC活性均有上升(P<0.01),而逍遥散组与模型组比较,突触体内Ca2+浓度及PKC活性则明显回落(P<0.05或P<0.01)。结论:慢性多相性应激可升高大鼠海马突触体内Ca2+浓度及PKC活性,逍遥散具有对抗应激升高大鼠海马突触体内Ca2+浓度及PKC活性的作用。
Objective： To observe the influence of Xiaoyao powder on the concentration of Ca^2＋ and PKC activity in hippoeampal synaptosome of multi stress model rat. Methods： Wistar rats were randomly allocated to five groups. All the rats except those of normal control were replicated to chronic multi-stress models. After the administration, the hippocampal homogenate was taken and loaded by Fura-2 ,the con centration of Ca^2＋ were detected by fluorirnetry and the PKC activity by agarose-gel electrophoresis. Resuits： Compared with normal control group, the concentration of Ca^2＋ and PKC activity in hippocampal synaptosome of both model groups were increased （ P 〈0.01） . Compared with model groups, both group of Xiaoyao powder were decreased （ P 〈0.05 or P 〈0.01）, Conclusion： These observation suggests multi-stress model can increase the concentration of Ca^2＋ and PKC activity in hippoeampal synaptosome of rats, and Xiaoyao powder can counteract such changes .
出处
《山东中医杂志》
2006年第2期112-114,共3页
Shandong Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.30271649)
国家中医药管理局资助项目(No.02-03JQ06)
广州中医药大学总体规划课题(No.K0C02015)
广州中医药大学创新基金课题(No.K004016)
关键词
逍遥散
心理应激
海马
钙离子
蛋白激酶C
Xiaoyan power
hippoeampal synaptosome
stress
Ca^2＋
Protein Kinase C
