摘要
【目的】探讨丹栀逍遥散原方及其提取物对大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、血浆皮质酮(CORT)含量的影响。【方法】Wistar大鼠随机分为6组:正常组、模型组、丹栀逍遥散高剂量组(剂量为14.4 g/kg)、复合物Ⅳ组(多糖高剂量组,2.20 g/kg)、复合物Ⅰ组(石油醚低剂量组,0.087 g/kg)、复合物Ⅱ组(水提醇沉液高剂量组,3.608 g/kg)。正常组灌服生理盐水不再施加任何刺激,模型组灌服生理盐水,采用Cart建立的慢性多相性应激模型并加以改进对大鼠进行造模,其他组灌服相对应的药物并造模,取血浆及下丘脑观察药物对大鼠下丘脑CRH、血浆CORT的影响,初步探索其作用机理。【结果】与正常组相比,模型组下丘脑CRH、血浆CORT含量升高;与模型组相比,复合物Ⅱ、复合物Ⅳ和丹栀逍遥散原方高剂量可改善由应激引起的下丘脑CRH含量升高情况(P<0.05或P<0.01);复合物Ⅰ、丹栀逍遥散原方高剂量组和复合物Ⅳ可降低血浆CORT值(P<0.05或P<0.01)。【结论】丹栀逍遥散及其提取物可以通过影响下丘脑CRH、血浆CORT水平参与对慢性心理应激反应的调控,CRH及CORT可能是它们抗慢性心理应激损伤的作用环节或靶点。
[ Objective] To investigate the effects of Dan Zhi Xiaoyao San （DZXS） and its extracts on hypothalamic corticotropin releasing hormone （CRH） and plasma corticosterone （CORT） in rats with chronic psychological stress. [Methods] Wistar rats were randomized into 6 groups： A （normal control）, B （model control）, C （extract of DZXS extracted by water extraction and alcohol precipitation, 3.608 g/kg）, D （extract of DZXS extracted by petroleum ether, 0.087 g/kg）, E （extract of DZXS extracted by polysaccharide, 2.20 g/kg） and F （DZXS 14.4 g/kg）. Except group A, the rat models of chronic multi-phase psychological stress were established in other groups by improved Cart method. Groups A and B were given normal saline, and groups C, D and E were given the corresponding drugs according to the experimentml design. Hypothalamic CRH and plasma CORT contents were examined to explore the therapeutic mechanism. [Results] Compared with group A, hypothalamic CRH and plasma CORT contents were increased in group B; hypothalamic CRH content was decreased in groups C, E and F （P 〈 0.05 or P 〈 0.01 ）, and plasma CORT content was decreased in groups D, E and F （P 〈 0.05 or P 〈 0.01）. [ Conclusion] DZXS and its extracts have an effect on chronic psychological stress by regulating hypothalamic CRH and plasma CORT contents, and CRH and CORT may be the pharmacological targets of DZXS in counteracting the damage induced by chronic psychological stress.
出处
《广州中医药大学学报》
CAS
2006年第5期413-415,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家中医药管理局课题(编号:02-03JQ06)
广州中医药大学总体规划课题(编号:KOC02015)
关键词
丹栀逍遥散/药理学
心理应激损伤/中药疗法
疾病模型
动物
大鼠
DAN ZHI XIAOYAO SAN/pharmacology
DAMAGE INDUCED BY PSYCHOLOGICAL STRESS/TCD therapy
DISEASE MODELS, ANIMAL
RATS
