摘要
目的制备单壁碳纳米管(SWNTs)靶向抗肿瘤药物载体,初步分析其药学特性和细胞学特性。方法功能化处理SWNTs后,在其表面负载各种生物活性分子,包括表面改性分子叶酸-聚乙二醇(PEG-FA)、抗肿瘤化疗药物阿霉素(DOX)以及荧光标记分子异硫氰酸荧光素(FITC),考察各生物分子负载情况及该药物载体的体外细胞学特性。结果酸处理及PEG-FA功能化后的SWNT能稳定分散于水中,长度为200~500 nm,浓度最高可达50μg.mL-1;DOX及FITC能在SWNTs表面稳定结合,最佳结合值pH7;药物载体能通过叶酸分子的靶向介导进入肿瘤细胞,较游离DOX有更强的体外抑癌效能。结论碳纳米管靶向抗肿瘤药物载体能靶向作用于肿瘤细胞,性状稳定,载药量可控,增加了化疗药物的抗癌活性。
OBJECTIVE To prepare a targeted antitumor drug delivery system with carbon nanotubes and study their pharmaceutical and in vitro cellular properties.METHODS Single-walled carbon nanotubes(SWNTs) were functionalized and loaded with different bioactive molecules including folate targeted polyethylene glycol(PEG-FA),antitumor drug adriamycin(DOX) and fluorescent labels fluorescein isothiocyanate(FITC).The binding efficiency of these molecules and the pharmaceutical and cellular properties were studied.RESULTS ...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第6期664-668,共5页
West China Journal of Pharmaceutical Sciences
基金
口腔疾病研究国家重点实验室自由申请项目(SKLODPD002)
四川大学青年基金(批准号:2008076)
关键词
碳纳米管
阿霉素
靶向
药物传递系统
抗癌活性
Carbon nanotube
Adriamycin
Targeted delivery
Drug delivery system
Antitumor activity
