摘要
为揭示应激对体内微量元素代谢的影响和机制,预先给予大鼠不同水平的铜3周后,采用冷束缚法使大鼠产生应激,测定了血清铜、锌水平及超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)和丙二醛(MDA)含量。结果表明,高铜给予没有对清洁级大鼠生长产生明显的影响,血清铜、锌及铜锌比值变化不明显,但适量的铜给予能显著提高血清中SOD活性和GSH含量;冷应激处理后大鼠血清中Cu水平下降,Zn水平升高,同时SOD消耗降低,而GSH含量显著升高。表明冷应激可使大鼠体内铜锌代谢及SOD活性和GSH含量发生变化,适量的铜给予在应激状况下才发挥出积极的生理作用。
To reveal the effects and mechanism of stress on metabolism of trace element in vivo,80 SD rats of 28 days old were divided into 4 groups at random,mamely feeding water group,low coper group(LCu),moderate copper group(MCu),and high copper group(HCu),feeding the four groups with deionized water lower,mod,and higher dose of copper respectively by intragastric administration for 3 weeks.Every group was randomly divided into ambient temperature contrasting groups and cold stress groups. Then observe and analyze concentration of serum copper and serum zinc,activity of superoside dismutase(SOD),reduced glutathionc(GSH)and malondialdehyde(MDA)of every group.The results showed that higher copper administration had not marked effects on the growth of clear SD rats.Serum copper/zinc ratio was not significantly changed.However,appropriate copper administration overly elevated activity of SOD and level of GSH.Serum copper decreased and serum zinc increased after the rats were disposed with cold stress.At the same time,SOD was consumed and level of GSH was notably heightened.Thus these results indicated that cold stress can change rats' metabolism of copper and zinc,activity of SOD and level of GSH,and appropriate administration of copper may make positive physiological effects in stress state.
出处
《广东微量元素科学》
CAS
2005年第4期18-22,共5页
Trace Elements Science
基金
温州医学院科技发展基金资助项目
