摘要
目的 观察铅对小鼠海马区c- fos表达及学习记忆功能的影响 ,并探讨二者的关系。方法 小鼠随机分为3个实验组和 1个对照组 ,每组 10只染铅剂量分别为 2 4、4 8和 9 6mmol L ,用跳台学习试验测试小鼠学习记忆能力 ;用RT PCR法观察各组小鼠海马区c fosmRNA的表达状况。结果 (1) 3个染铅组小鼠跳台学习成绩显著低于对照组 (P <0 . 0 1) ;(2 ) 3个染铅组小鼠c- fosmRNA水平均明显降低 ,以对照组的值为 10 0 ,则低剂量组为 75 . 0 4± 4 .75 ,中剂量组为5 8. 3 5± 5 . 2 9,高剂量组为 42. 66± 6 .3 0 ,与对照组相比 ,P <0 .0 1。高剂量组、中剂量组与低剂量组比较 ,P <0 . 0 1。结论 慢性铅染毒间接阻碍了c- fox基因的反应性表达 ,c- fos基因表达水平的下降可能与铅致小鼠学习记忆功能损害有关。
Objective To observe the effect of lead exposure on the capacity of learning and memory an d on the expression of c-fos mRNA in hippocampus of mice.Methods Acetic lead was applied on mice at the dose of (2.4, 4.8, 9.6 mmol/L) for 6 we eks.The step down test was used to measure the capacity of learning and memory o f each group.At the end of the experiment,RT-PCR was used to observe the expres sion of c-fos mRNA in hippocampus after the induction of step down test.Results (1)In step down test,the correct precentage of lead groups si gnificantly decreased compared with the controls (P<0.01).(2)Results of c-fos RT-PCR:The expression of c-fos in hippocampus of control groups was 10%.The expression of c-fos in hippocampus of low lead groups was 75. 04±4.75;and it was 58.35±5.29 in middle lead groups;was 42.66±6.30 in low lea d groups.Compared with control groups,the others groups had significanceP<0.01.Compared wit h low lead gorups,middle and high lead groups had significanceP<0.01.Conclusion The results suggested that the decrease of c-fos gene e xpression level in hippocampus could associate with the impairment of learning a nd memory capacity in mice induced by lead.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2005年第1期21-23,共3页
Journal of Toxicology
基金
国家自然科学基金 (39970 651 )
