摘要
目的:观察大鼠慢性脑缺血所致的空间学习记忆功能损害及促红细胞生成素(EPO)对其认知功能的影响。方法:结扎大鼠双侧颈总动脉建立慢性脑缺血模型,治疗组术后给予EPO1000U/kg,持续5d。术后第8周时3组大鼠Morris水迷宫测试后断头取脑做组织学和流式细胞术检测。结果:缺血组水迷宫表现同假手术组和EPO治疗组相比有显著差异(P<0.05)。EPO能改善慢性脑缺血大鼠的空间学习记忆能力(P<0.05)。EPO组神经细胞病理改变较轻,海马细胞凋亡率较低(P<0.01)。结论:EPO可能通过减少海马CA1区神经元的损害以及抗凋亡作用改善慢性脑缺血大鼠空间学习记忆障碍。
Objective： To study the injury of spatial learning and memory Oability in rats with chronic cerebral hypoperfusion and the protective effects of erythropoietn（EPO） on the rats＇ cognitive function. Methods： The chronic cerebral hypoperfusion models were made by permanent bilateral occlusion of both common carotid arteries （2-VO methods）. The EPO-treatment group received EPO（1 000 U/kg） for five days after the operation, while the other two groups were received equal volume PBS. Spatial learning and memory abilities were evaluated with Morris water maze at the 8th week after the operation. Then the rats＇ brains were removed for histopathological and flow cytometry evaluation. Results： The escape latencies in operation rats were significantly prolonged （P〈 0.05） ; and the times of passing through the platform were significantly decreased than the other groups （both P〈 0.05）. EPO treatment significantly improved spatial learning and memory abilities of chronic cerebral hypoperfusion rats （P〈 0.05）, and alleviated the brain injury and neuronal apoptosis in hippocampus （P〈 0.01）. Conclusion： EPO can reduce to improve hippocampal CAl neurons loss and alleviate neuronal apoptosis in hippocampus of rat the spatial learning and memory abilities.
出处
《武汉大学学报（医学版）》
CAS
2006年第2期225-228,F0003,共5页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30270483)
