摘要
目的 研究线粒体、内质网Ca2 + ATP酶以及Na+ 和K+ ATP酶活力的改变对铅引起的 [Ca2 + ] i 升高的调节作用 ,探讨铅引起的 [Ca2 + ] i 增高对学习记忆功能的影响。方法 Wistar大鼠神经肉瘤C6 细胞培养于含醋酸铅的培养液中 ,于不同时间终止染铅 ,检测 [Ca2 + ] i 及线粒体、内质网Ca2 + ATP酶、Na+ 和K+ ATP酶活力。结果 (1) 0 2 μmol L染铅组 :[Ca2 + ] i 轻度升高 ,线粒体Ca2 + ATP酶、Na+ 和K+ ATP酶活力升高 ,内质网酶活力略增高 ;(2 ) 1 0 μmol L染铅组 :[Ca2 + ] i 于染铅后 0 5h升至最高 ,以后逐渐降低 ,波动于 160～ 190nmol L之间 ;线粒体、内质网Ca2 + ATP酶、Na+ 和K+ ATP酶于染铅后 0 5h升至最高 (分别为未染铅前酶活力的 2 9 1、3 9 2、10 8和 19 8倍 ) ,2d后降至接近正常水平。结论(1)铅可使Wistar大鼠神经肉瘤C6 细胞Ca2 + 浓度及线粒体、内质网Ca2 + ATP酶、Na+ 和K+ ATP酶活力增高 ;(2 )当[Ca2 + ] i 增高不显著时以线粒体Ca2 + ATP酶、Na+ 和K+ ATP酶活力增高为主 ;当 [Ca2 + ] i 急剧升高时 ,线粒体、内质网Ca2 + ATP酶、Na+ 和K+ ATP酶活力均明显增高 ,尤其是线粒体Ca2 + ATP酶、Na+ 和K+ ATP酶活力增高更为显著。
Objective In order to explore the effects of the elevation of [Ca 2+ ]\-i on learning and memory,we study the regulation effects of Ca 2+ -ATPase,Na\++,K\++-ATPase in mitochondrion and endoplasmic reticulum on the elevation of [Ca 2+ ]\-i induced by lead.Methods C\-6 cells of Wistar rat neural sarcoma were cultured in DMEM culture solution containing acetate lead for various time.[Ca 2+ ]\-i,Ca 2+ -ATPase and Na\++,K\++-ATPase activity of mitochondrion and endoplasmic reticulum were determined.Results (1)group of 0\^2 μmol/L lead acetate:[Ca 2+ ]\-i increased slightly,Ca 2+ -ATPase and Na +,K +-ATPase activity of mitochondrion increased,and those of endoplasmic reticulum also increased slightly;(2)group of 1\^0 μmol/L lead acetate:[Ca 2+ ]\-i increased and reached the highest level after 0\^5 h,then decreased gradually and changed bteween 160 nmol/L and 190 nmol/L.Ca 2+ -ATPase and Na\++,K\++-ATPase activity of mitochondrion and endoplasmic reticulum reached the highest level after 0\^5 h(29\^1,39\^2,10\^8 and 19\^8 times as high as the activity before exposed to lead respectively),then decreased to normal level after 2days. Conclusion (1)[Ca 2+ ]\-i,Ca 2- -ATPase and Na\++,K\++-ATPase activity of mitochondrion and endoplasmic reticulum increased in C\-6 cells of rat neural sercoma exposed to lead acetate;(2)when elevation of [Ca 2+ ]\-i is unobvious,Ca 2+ -ATPase and Na\++,K\++-ATPase activity increased mainly in mitochondrion;and when [Ca 2+ ]\-i increased sharply,Ca 2+ -ATPase and Na\++,K\++-ATPase activity increased in both mitochondrion and endoplasmic reticulum,but the changes in mitochondrion is more obvjous.
出处
《卫生毒理学杂志》
CSCD
北大核心
2003年第2期69-71,共3页
Journal of Health Toxicology
基金
国家自然科学基金课题 (39970 651 )
