KangFuXinYe(KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for ma...KangFuXinYe(KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China.However,its therapeutic effect and mechanism are not yet well understood.Thus,the aim of this study was to investigate the gastroprotective effects of KangFuXinYe(KFX)in indomethacin-induced gastric damage.Rats were randomly divided into six groups as follows:control,treated with indomethacin(35 mg·kg^-1),different dosages of KFX(2.57,5.14 and 10.28 mL·kg^-1,respectively)plus indomethacin,and sucralfate(1.71 mL·kg^-1)plus indomethacin.After treatment,rat serum,stomach and gastric homogenates were collected for biochemical tests and examination of histopathology firstly.Rat serum was further used for metabolomics analysis to research possible mechanisms.Our results showed that KFX treatment alleviated indomethacin-induced histopathologic damage in rat gastric mucosa.Meanwhile,its treatment significantly increased cyclooxygenase-1(COX-1),prostaglandin E2(PGE2)and epidermal growth factor(EGF)levels in rat serum and gastric mucosa.Moreover,KFX decreased cyclooxygenase-2(COX-2)and interleukin-6(IL-6)levels.Nine metabolites were identified which intensities significantly changed in gastric damage rats,including 5-hydroxyindoleacetic acid,indoxylsulfuric acid,indolelactic acid,4-hydroxyindole,pantothenic acid,isobutyryl carnitine,3-methyl-2-oxovaleric acid,sphingosine 1-phosphate,and indometacin.These metabolic deviations came to closer to normal levels after KFX intervention.The results indicate that KFX(10.28 mL·kg^-1)exerts protective effects on indomethacin-induced gastric damage by possible mechanisms of action(regulating tryptophan metabolism,protecting the mitochondria,and adjusting lipid metabolism,and reducing excessive indomethacin).展开更多
目的观察柳氮磺胺吡啶联合康复新液治疗溃疡性结肠炎的临床效果。方法选择2014年5月至2016年5月本院收治的溃疡性结肠炎患者82例作为研究对象,随机分为观察组与对照组,每组各41例。对照组予柳氮磺胺吡啶治疗,观察组在对照组基础上联合...目的观察柳氮磺胺吡啶联合康复新液治疗溃疡性结肠炎的临床效果。方法选择2014年5月至2016年5月本院收治的溃疡性结肠炎患者82例作为研究对象,随机分为观察组与对照组,每组各41例。对照组予柳氮磺胺吡啶治疗,观察组在对照组基础上联合康复新液。比较两组治疗前后的血清降钙素原(PCT)、C反应蛋白(CRP)、肠道黏膜核因子(NF)-κB与Toll样受体4(TLR-4)含量、疗效以及不良反应总发生率。结果两组治疗前血清PCT与CRP水平、NF-κB与TLR-4 m RNA含量的差异均无统计学意义(P>0.05);治疗后观察组血清PCT、CRP水平为(0.44±0.10)ng/m L、(14.56±4.20)mg/m L,NF-κB、TLR-4 m RNA含量为(36.54±6.77)、(38.69±5.86),均低于本组治疗前与对照组治疗后同一时点(均P<0.05)。观察组有效率为90.24%,高于对照组的73.17%,差异有统计学意义(P<0.05)。观察组及对照组不良反应总发生率分别为14.63%、9.76%,差异无统计学意义(P>0.05)。结论相比于单用柳氮磺胺吡啶,联用康复新液的治疗方案更利于降低溃疡性结肠炎患者的血清PCT、CRP水平以及肠道黏膜NF-κB及TLR-4的表达,临床疗效更优。展开更多
文摘KangFuXinYe(KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China.However,its therapeutic effect and mechanism are not yet well understood.Thus,the aim of this study was to investigate the gastroprotective effects of KangFuXinYe(KFX)in indomethacin-induced gastric damage.Rats were randomly divided into six groups as follows:control,treated with indomethacin(35 mg·kg^-1),different dosages of KFX(2.57,5.14 and 10.28 mL·kg^-1,respectively)plus indomethacin,and sucralfate(1.71 mL·kg^-1)plus indomethacin.After treatment,rat serum,stomach and gastric homogenates were collected for biochemical tests and examination of histopathology firstly.Rat serum was further used for metabolomics analysis to research possible mechanisms.Our results showed that KFX treatment alleviated indomethacin-induced histopathologic damage in rat gastric mucosa.Meanwhile,its treatment significantly increased cyclooxygenase-1(COX-1),prostaglandin E2(PGE2)and epidermal growth factor(EGF)levels in rat serum and gastric mucosa.Moreover,KFX decreased cyclooxygenase-2(COX-2)and interleukin-6(IL-6)levels.Nine metabolites were identified which intensities significantly changed in gastric damage rats,including 5-hydroxyindoleacetic acid,indoxylsulfuric acid,indolelactic acid,4-hydroxyindole,pantothenic acid,isobutyryl carnitine,3-methyl-2-oxovaleric acid,sphingosine 1-phosphate,and indometacin.These metabolic deviations came to closer to normal levels after KFX intervention.The results indicate that KFX(10.28 mL·kg^-1)exerts protective effects on indomethacin-induced gastric damage by possible mechanisms of action(regulating tryptophan metabolism,protecting the mitochondria,and adjusting lipid metabolism,and reducing excessive indomethacin).
文摘目的观察柳氮磺胺吡啶联合康复新液治疗溃疡性结肠炎的临床效果。方法选择2014年5月至2016年5月本院收治的溃疡性结肠炎患者82例作为研究对象,随机分为观察组与对照组,每组各41例。对照组予柳氮磺胺吡啶治疗,观察组在对照组基础上联合康复新液。比较两组治疗前后的血清降钙素原(PCT)、C反应蛋白(CRP)、肠道黏膜核因子(NF)-κB与Toll样受体4(TLR-4)含量、疗效以及不良反应总发生率。结果两组治疗前血清PCT与CRP水平、NF-κB与TLR-4 m RNA含量的差异均无统计学意义(P>0.05);治疗后观察组血清PCT、CRP水平为(0.44±0.10)ng/m L、(14.56±4.20)mg/m L,NF-κB、TLR-4 m RNA含量为(36.54±6.77)、(38.69±5.86),均低于本组治疗前与对照组治疗后同一时点(均P<0.05)。观察组有效率为90.24%,高于对照组的73.17%,差异有统计学意义(P<0.05)。观察组及对照组不良反应总发生率分别为14.63%、9.76%,差异无统计学意义(P>0.05)。结论相比于单用柳氮磺胺吡啶,联用康复新液的治疗方案更利于降低溃疡性结肠炎患者的血清PCT、CRP水平以及肠道黏膜NF-κB及TLR-4的表达,临床疗效更优。
