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玫瑰香干红葡萄酒自然发酵过程中优势酵母分离鉴定及其应用潜力分析 被引量:18
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作者 阎贺静 时月 +1 位作者 刘畅 赵琳琳 《食品科学》 EI CAS CSCD 北大核心 2017年第22期117-124,共8页
为酿造特色玫瑰香干红葡萄酒,开发本土发酵剂是目前重要发展方向。从昌黎产区玫瑰香干红葡萄酒自然发酵醪中共分离得到337株酵母,通过WL(Wallerstein laboratory)鉴别培养基对其进行分类鉴定、计数,将其鉴别为7大类,根据计数结果选取优... 为酿造特色玫瑰香干红葡萄酒,开发本土发酵剂是目前重要发展方向。从昌黎产区玫瑰香干红葡萄酒自然发酵醪中共分离得到337株酵母,通过WL(Wallerstein laboratory)鉴别培养基对其进行分类鉴定、计数,将其鉴别为7大类,根据计数结果选取优势酵母接种至BIGGY(bismuth sulphite glucose glycine yeast)固体培养基,从中进一步筛选不产H2S酵母,进行分子鉴定,结果显示HBKS-Y1、HBKS-Y3为酿酒酵母,HBKS-Y2为葡萄汁有孢汉逊酵母。考察3株本土酵母酿造因子耐性,发现3株酵母对乙醇、SO2、糖度均具有较高耐性,满足葡萄酒酿造要求;以商用活性干酵母FX10葡萄汁发酵为对照,考察3株酵母对玫瑰香葡萄汁单菌、混菌发酵理化指标和香气成分的影响,3株本土酵母单菌和混菌发酵各指标(例如:还原糖、酒精度、总酸、挥发酸、甘油和乙醛含量)均符合干红葡萄酒酿造标准。同时发现,与HBKS-Y2单菌发酵相比,3株酵母混菌发酵总酸和挥发酸含量降低,对葡萄酒有益,且混菌发酵对葡萄酒其他理化指标没有不利影响。本土酵母发酵尤其是混菌发酵,表征玫瑰香品种香气的萜烯醇类物质含量明显高于活性干酵母发酵。以上结果表明,3株本土酵母可以应用于玫瑰香干红葡萄酒的酿造,且3株酵母混菌发酵对突出玫瑰香干红葡萄酒品种香气具有一定的应用潜力。 展开更多
关键词 玫瑰香干红葡萄酒 本土酵母 分离 鉴定 酿造特性 混菌发酵
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Vehicle Re-Identication Model Based on Optimized DenseNet121 with Joint Loss 被引量:12
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作者 Xiaorui Zhang Xuan Chen +1 位作者 Wei Sun Xiaozheng He 《Computers, Materials & Continua》 SCIE EI 2021年第6期3933-3948,共16页
With the increasing application of surveillance cameras,vehicle re-identication(Re-ID)has attracted more attention in the eld of public security.Vehicle Re-ID meets challenge attributable to the large intra-class diff... With the increasing application of surveillance cameras,vehicle re-identication(Re-ID)has attracted more attention in the eld of public security.Vehicle Re-ID meets challenge attributable to the large intra-class differences caused by different views of vehicles in the traveling process and obvious inter-class similarities caused by similar appearances.Plentiful existing methods focus on local attributes by marking local locations.However,these methods require additional annotations,resulting in complex algorithms and insufferable computation time.To cope with these challenges,this paper proposes a vehicle Re-ID model based on optimized DenseNet121 with joint loss.This model applies the SE block to automatically obtain the importance of each channel feature and assign the corresponding weight to it,then features are transferred to the deep layer by adjusting the corresponding weights,which reduces the transmission of redundant information in the process of feature reuse in DenseNet121.At the same time,the proposed model leverages the complementary expression advantages of middle features of the CNN to enhance the feature expression ability.Additionally,a joint loss with focal loss and triplet loss is proposed in vehicle Re-ID to enhance the model’s ability to discriminate difcult-to-separate samples by enlarging the weight of the difcult-to-separate samples during the training process.Experimental results on the VeRi-776 dataset show that mAP and Rank-1 reach 75.5%and 94.8%,respectively.Besides,Rank-1 on small,medium and large sub-datasets of Vehicle ID dataset reach 81.3%,78.9%,and 76.5%,respectively,which surpasses most existing vehicle Re-ID methods. 展开更多
关键词 Vehicle re-identication densenet joint loss focal loss SE block
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云南草坪地被植物真菌病害研究(六) 被引量:4
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作者 张陶 王革 +3 位作者 桂明英 张中义 刘云龙 崔瑛 《云南农业大学学报》 CAS CSCD 2000年第3期222-224,共3页
报道云南草坪地被植物真菌病害 1 1种 ,其中 :国内新记录 3种 ,省内新记录 4种 ,省内已知种新分布 4种。前二者有简要描述 ,后者列名录 ,供植检和植保工作参考。
关键词 草坪地被植物 真菌病害 新记录种 新分布种
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A Continuous Time Domain Direct Identication Method for Unsteady Fluid Dynamic Model in Incompressible Flow 被引量:1
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作者 Xianwu Lin Tao Li +1 位作者 Lixun Xiong Delin Luo 《Guidance, Navigation and Control》 2023年第1期85-116,共32页
To identify the unsteady°uid dynamic model for a solid moving in the°uid,a new identication method that operates in the continuous time domain is proposed.It is illustrated that this new identication method ... To identify the unsteady°uid dynamic model for a solid moving in the°uid,a new identication method that operates in the continuous time domain is proposed.It is illustrated that this new identication method is more accurate and appropriate for identifying unsteady°uid dynamic model in incompressible°ow in comparison with the classical frequency domain or discrete domain method.This new method is applied to identify the unsteady°uid dynamic models of a NACA0012 airfoil and the e®ect of the°uid viscosity on the model parameter is analyzed.The result shows that the added mass in viscous°ow is dependent on the input signal frequency and is exactly the added mass in inviscid°ow when the frequency approaches innity.Based on this discovery,the long controversy on the relationship of added mass between viscous and inviscid°ow is solved.Then,the enlightenment of this discovery on the identication of both linear and nonlinear unsteady aerodynamic model in incompressible°ow is illustrated.At last,the advantage of the new acquired unsteady°uid dynamic model is discussed in comparison with the classical quasi-steady aerodynamic model. 展开更多
关键词 Model identication unsteady fluid dynamics added mass non-causal system.
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16S rDNA鉴定泡椒凤爪中的腐败菌 被引量:1
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作者 孙玉侠 黄国保 《食品安全导刊》 2023年第6期73-75,共3页
泡椒凤爪是我国西南泡椒肉系列工业化产品中的代表性产品。本文采用传统分离培养结合16S rDNA分子生物学方法,鉴定引起泡椒凤爪胀袋变质的微生物。结果显示,引起腐败的主要是葡萄球菌、芽孢杆菌、假单胞菌。本文根据特定腐败菌的生物学... 泡椒凤爪是我国西南泡椒肉系列工业化产品中的代表性产品。本文采用传统分离培养结合16S rDNA分子生物学方法,鉴定引起泡椒凤爪胀袋变质的微生物。结果显示,引起腐败的主要是葡萄球菌、芽孢杆菌、假单胞菌。本文根据特定腐败菌的生物学特性,提出了相关建议,以期延长泡椒凤爪的货架期。 展开更多
关键词 泡椒凤爪 16S rDNA 腐败菌 鉴定
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Blind channel identication of nonlinear folding mixing model 被引量:1
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作者 Su Yong Xu Shangzhi Ye Zhongfu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第3期509-512,共4页
Signals from multi-sensor systems are often mixtures of (statistically) independent sources by unknown mixing method. Blind source separation(BSS) and independent component analysis(ICA) are the methods to ident... Signals from multi-sensor systems are often mixtures of (statistically) independent sources by unknown mixing method. Blind source separation(BSS) and independent component analysis(ICA) are the methods to identify/recover the channels and the sources. BSS/ICA of nonlinear mixing models are difficult problems. For instance, the post-nonlinear model has been studied by several authors. It is noticed that in most cases, the proposed models are always with an invertible mixing. According to this fact there is an interesting question, how about the situation of the non-invertible non-linear mixing in BSS or ICA? A new simple non-linear mixing model is proposed with a kind of non-invertible mixing, the folding mixing, and method to identify its channel, blindly. 展开更多
关键词 BLIND channel identication non-linear mixing non-invertible.
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鹅源禽痘病毒的鉴定和基因分析 被引量:4
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作者 张红云 郑敏 +4 位作者 罗满林 曹慧慧 孙文超 韦显凯 闭璟珊 《病毒学报》 CAS CSCD 北大核心 2017年第6期892-897,共6页
2016年6月,广西邕宁某场有20%的阳江鹅在喙、眼和脚出现赘肉状痘癍,临床无死亡病例。为了研究此病的病原和基因特征,采集痘癍样本、制备病理组织切片进行显微镜观察、并利用透射电镜进行形态学分析以及TaqMan荧光定量PCR检测,证实其病... 2016年6月,广西邕宁某场有20%的阳江鹅在喙、眼和脚出现赘肉状痘癍,临床无死亡病例。为了研究此病的病原和基因特征,采集痘癍样本、制备病理组织切片进行显微镜观察、并利用透射电镜进行形态学分析以及TaqMan荧光定量PCR检测,证实其病原为禽痘病毒属(Avipoxvirus,APV)成员,命名为YNE。通过对病毒核芯蛋白P4b编码基因(fpv167)和DNA聚合酶Popr编码基因(fpv094)的部分序列串联进行比对,发现该毒株与1991年分离于北美林鸳鸯的APV高度同源,属于A5.2基因分支。进一步序列分析发现,该毒株与同期发生在该场的麻鸭源APV相应序列的同源性可达99.9%,初步证实该病毒可以在鸭鹅间跨种传播。 展开更多
关键词 禽痘病毒(APV) 水禽 鉴定 分型
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Identification and evaluation of fault-fracture reservoirs in buried hills of the Lower Paleozoic, Chengdao area, China
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作者 Zhiwei Wang Kai Zhang +1 位作者 Yuhan Cheng Qunhu Wu 《Energy Geoscience》 2023年第3期193-202,共10页
The Bohai Bay Basin is a Meso-Cenozoic rifted basin where the Paleozoic buried hills with great hydrocarbon potentials are well developed. The reservoir space types are complex and diverse due to tectonic activities, ... The Bohai Bay Basin is a Meso-Cenozoic rifted basin where the Paleozoic buried hills with great hydrocarbon potentials are well developed. The reservoir space types are complex and diverse due to tectonic activities, making fracture distribution highly heterogeneous. Reservoir identification and mapping is challenging due to their large burial depth and poor resolution of seismic data. An integration of well-logging, seismic data interpretation and core observation is applied to identify three structural unit types in the study area, that is, fault breccia zone, fault cataclastic zone, and fault massive rock zone. A comprehensive well-logging identification template and a comprehensive discriminant function M for the reservoir are established based on the well-logging response characteristics. A M value greater than 0.12 indicates a fault breccia zone, that between 0.04 and 0.12 marks a fault cataclastic zone, and that in the range from 0.02 to 0.04 represents a fault massive rock zone. A seismic prediction method with multi-parameter fusion is proposed in the study. The large-scale fractures are mapped by coherence-clutter parameters, while small fractures are predicted via waveform indication inversion. The spatial distribution of “fault-fracture reservoirs” is precisely mapped by frequency fusion technology. It is found that the fault breccia zones usually occur close to the fault planes, while the fault cataclastic zones are slightly away from the fault planes. The hydrocarbon abundance of the breccia zones is greater than that of the fault cataclastic and fault massive rock zones. 展开更多
关键词 Buried hill Fault-facture Ieservoir Reservoir logging identication Reservoir sei smnic mapping Lower paleozoic Chengdao area
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复方苦黄喷雾剂的TCL鉴定研究 被引量:2
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作者 刘龙友 陈青燕 汤迎爽 《中医药导报》 2012年第12期82-84,共3页
目的:建立复方苦黄喷雾剂的薄层鉴别方法。方法:采用TLC法对处方中黄柏、大黄、冰片等进行定性鉴别。结果:薄层色谱斑点清晰,阴性无干扰。结论该方法简便可行,可用于复方苦黄喷雾剂的质量控制。
关键词 复方苦黄喷雾剂 薄层色谱法 鉴别
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甜瓜果实中芳香物质及香气鉴定技术应用研究 被引量:2
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作者 刘永刚 刘敦华 王锐 《安徽农学通报》 2018年第23期10-12,67,共4页
该文综述了甜瓜不同生理期的芳香物质种类及含量变化,并对电子鼻、气相色谱-质谱联用技术、气相色谱-嗅觉辨别技术在甜瓜香气成分分析领域的应用予以阐述,以期为甜瓜香气的进一步研究奠定基础。
关键词 甜瓜 芳香物质 香气 鉴定技术
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白木香聚酮环化酶基因AsPKC1的克隆及表达分析 被引量:2
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作者 马纳纳 丁旭坡 +2 位作者 蔡彩虹 戴好富 梅文莉 《分子植物育种》 CAS CSCD 北大核心 2020年第5期1472-1479,共8页
聚酮环化酶是植物聚酮合成途径中的关键酶之一,可催化CoA类前体直接合成多环结构。作为聚酮合成下游产物的2-(2-苯乙基)色酮是名贵中药材沉香形成的重要标志物之一,聚酮环化酶可能在其形成过程中发挥着重要作用。本研究基于白木香结香... 聚酮环化酶是植物聚酮合成途径中的关键酶之一,可催化CoA类前体直接合成多环结构。作为聚酮合成下游产物的2-(2-苯乙基)色酮是名贵中药材沉香形成的重要标志物之一,聚酮环化酶可能在其形成过程中发挥着重要作用。本研究基于白木香结香转录组数据,首次克隆了1个白木香聚酮环化酶基因,命名为AsPKC1,该基因的编码区共含1 116个核苷酸,编码371个氨基酸残基。AsPKC1编码的蛋白序列与已报道的PKC蛋白高度相似,与锦葵科棉花、榴莲和可可树具有较近的亲缘关系,聚于进化树的同一分支;该蛋白的等电点为9.04,分子量为40.43 kD,包含3个糖基化位点和28个磷酸化位点,为非分泌型蛋白,α螺旋和无规则卷曲组成其主要二级结构。实时荧光定量PCR验证实验表明AsPKC1的表达具有组织特异性,并且在结香茎干中持续上调表达,其第9天在白木香茎干中的表达量为对照组的6.52倍。本研究为进一步解析聚酮环化酶基因在沉香标志物2-(2-苯乙基)色酮合成及白木香结香过程中的作用提供了数据支持。 展开更多
关键词 白木香(Aquliaria sinensis) 聚酮环化酶 鉴定 生物信息学 表达
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无绿藻研究进展 被引量:2
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作者 赵悦 朱巍巍 +4 位作者 刘锦燕 李文静 王影 史册 项明洁 《检验医学》 CAS 2017年第10期97-101,共5页
无绿藻是一种广泛存在于自然界的条件致病菌,感染动物时主要引起乳腺炎,对人的感染则主要表现为皮肤感染、鹰嘴滑囊炎和系统性感染。目前临床上仍将镜检和生化反应作为鉴定该菌的主要方法,但由于耗时长,主观影响较大,加之国内尚对无绿... 无绿藻是一种广泛存在于自然界的条件致病菌,感染动物时主要引起乳腺炎,对人的感染则主要表现为皮肤感染、鹰嘴滑囊炎和系统性感染。目前临床上仍将镜检和生化反应作为鉴定该菌的主要方法,但由于耗时长,主观影响较大,加之国内尚对无绿藻的认识不足,导致该菌的检出率较低。为提高对无绿藻的认识,文章对其生物学特性、致病性、流行病学特征、鉴定、耐药性及无绿藻病的临床表现等作一综述。 展开更多
关键词 无绿藻 无绿藻病 内孢子 条件致病菌 鉴定 基因型
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Reconstructing the Time-Dependent Thermal Coefcient in 2D Free Boundary Problems
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作者 M.J.Huntul 《Computers, Materials & Continua》 SCIE EI 2021年第6期3681-3699,共19页
The inverse problem of reconstructing the time-dependent thermal conductivity and free boundary coefcients along with the temperature in a two-dimensional parabolic equation with initial and boundary conditions and ad... The inverse problem of reconstructing the time-dependent thermal conductivity and free boundary coefcients along with the temperature in a two-dimensional parabolic equation with initial and boundary conditions and additional measurements is,for the rst time,numerically investigated.This inverse problem appears extensively in the modelling of various phenomena in engineering and physics.For instance,steel annealing,vacuum-arc welding,fusion welding,continuous casting,metallurgy,aircraft,oil and gas production during drilling and operation of wells.From literature we already know that this inverse problem has a unique solution.However,the problem is still ill-posed by being unstable to noise in the input data.For the numerical realization,we apply the alternating direction explicit method along with the Tikhonov regularization to nd a stable and accurate numerical solution of nite differences.The root mean square error(rmse)values for various noise levels p for both smooth and non-smooth continuous time-dependent coef-cients Examples are compared.The resulting nonlinear minimization problem is solved numerically using the MATLAB subroutine lsqnonlin.Both exact and numerically simulated noisy input data are inverted.Numerical results presented for two examples show the efciency of the computational method and the accuracy and stability of the numerical solution even in the presence of noise in the input data. 展开更多
关键词 Inverse identication problem two-dimensional parabolic equation free boundary Tikhonov regularization nonlinear optimization
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小鼠CD4^+T细胞外泌体分离及鉴定
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作者 张伟鑫 曾仲 《中国比较医学杂志》 北大核心 2017年第10期60-64,共5页
目的探讨CD4^+T细胞是否具有分泌外泌体的能力,以及CD4^+T细胞外泌体参与炎症及肿瘤免疫过程的可能。方法应用断颈处死的方法取出小鼠的脾脏,用磁珠分选的方法从小鼠脾脏细胞中分选出CD4^+T细胞,并尝试从培养基上清液中提取CD4^+T细胞... 目的探讨CD4^+T细胞是否具有分泌外泌体的能力,以及CD4^+T细胞外泌体参与炎症及肿瘤免疫过程的可能。方法应用断颈处死的方法取出小鼠的脾脏,用磁珠分选的方法从小鼠脾脏细胞中分选出CD4^+T细胞,并尝试从培养基上清液中提取CD4^+T细胞的外泌体,在透射电镜下观察,后用PCR技术分析miR-23a、miR-214的表达情况。结果在透射电镜下能够观察到提取出的CD4^+T细胞外泌体,且用PCR技术能检测到小鼠CD4^+T细胞外泌体中miR-23a、miR-214这两个炎症及肿瘤相关的miRNA的表达。结论小鼠CD4^+T细胞有分泌外泌体的能力,且为CD4^+T细胞外泌体参与炎症及肿瘤相关过程提供了可能。 展开更多
关键词 CD4+T细胞 外泌体 分离 鉴定 MIRNA
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葡萄砧木品种‘3309M’中microRNA的鉴定
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作者 朱旭东 安颜颖 +2 位作者 王晨 贾海锋 房经贵 《中外葡萄与葡萄酒》 2018年第3期6-15,共10页
为了解葡萄砧木microRNA(miRNA)的表达模式,采用高通量测序法对砧木品种‘3309M’根和叶片中的miRNA文库进行分析。结果表明:在葡萄砧木根和叶片文库中,共鉴定了181条保守miRNA和104条新的miRNA,长度以21 nt为主,其5'端首位碱基对U... 为了解葡萄砧木microRNA(miRNA)的表达模式,采用高通量测序法对砧木品种‘3309M’根和叶片中的miRNA文库进行分析。结果表明:在葡萄砧木根和叶片文库中,共鉴定了181条保守miRNA和104条新的miRNA,长度以21 nt为主,其5'端首位碱基对U具有很强的偏向性;不同miRNA家族其成员数量也不同,这些miRNA的靶基因功能主要为植物激素信号转导和糖酵解/糖异生等;筛选出显著差异表达的miRNA,其中54条miRNA表达量上调,33条miRNA表达量下调。本研究为认识葡萄砧木特异miRNA及进一步分析其功能提供了基础。 展开更多
关键词 葡萄 砧木 3309M MIRNA 靶基因 鉴定
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浅议Cd^(2+)的鉴定
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作者 陈美珠 郑惠榕 《闽江学院学报》 2000年第3期38-40,共3页
本文探讨了在硫化氢系统分析法中 ,鉴定Cd2 + 出现的一些问题 ,提出了Cd2 +
关键词 Cd2+ 酸度 鉴定 沉淀
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结构向量自回归因果图的性质及应用
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作者 魏岳嵩 《淮北师范大学学报(自然科学版)》 CAS 2015年第4期1-4,共4页
文章利用图模型方法分析结构向量自回归模型变量间的因果性问题,构建结构向量自回归因果图,研究该因果图的性质,基于信息论方法建立了因果图结构辨识的三步准则,并用所给方法做了实例分析.
关键词 向量自回归模型 因果图 互信息 辨识
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用原子吸收分光光度法鉴别陶瓷品
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作者 姚中栋 汪建君 +1 位作者 奚建华 裘贞庭 《法医学杂志》 CAS CSCD 1994年第1期18-21,共4页
陶瓷制品的鉴别是司法鉴定实践中的一个重要课题.用原子吸收分光光度法精确测定陶瓷制品中Pb、Zr、Fe、Zn等金属元素的含量.实验结果表明.不仅可区别不同类型的陶瓷制品,而且可区别不同地区或不同工厂生产的陶瓷制品.
关键词 原子吸收分光光度法 鉴别 陶瓷品 微量金属元素 含量 法医物证检验学
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Hacking Anti-Shoplifting System to Hide Data within Clothes
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作者 Al Hussien Seddik Saad E.H.Hafez Zubair Ahmad 《Computers, Materials & Continua》 SCIE EI 2021年第4期659-674,共16页
Steganography has been used to prevent unauthorized access to private information during transmission.It is the scheme of securing sensitive information by concealing it within carriers such as digital images,videos,a... Steganography has been used to prevent unauthorized access to private information during transmission.It is the scheme of securing sensitive information by concealing it within carriers such as digital images,videos,audio,or text.Current steganography methods are working by assigning a cover le then embed the payload within it by making some modications,creating the stego-le.However,the left traces that are caused by these modications will make steganalysis algorithms easily detect the hidden payload.Aiming to solve this issue,a novel,highly robust steganography method based on hacking anti-shoplifting systems has proposed to hide data within clothes.The anti-Shoplifting system is an anti-theft security system that protects goods and products,leaving the store in an illegal way(i.e.,without paying for them).The proposed method works by modifying the default anti-shoplifting system by changing its built-in soft RFID(radio-frequency identication)tags sewn in clothes into NFC(Near Field Communication)tags.These NFC tags are smart tags that can communicate with NFC-Enabled smart-phones using NDEF(NFC Data Exchange Format).NDEF is one of the advancements added to RFID technology by NFC,which allows the data exchange.Every NDEF message has one/more NDEF records that contain record type,a unique ID,a length,and a payload of data that contains the secret message content that can be any type of data that ts in a byte stream.Based on NDEF and NFC-enabled smart-phones,the proposed method will take the secret message from the sender,make use of his NFC-enabled smart-phone to communicate with the NFC tag,then hide the secret message within the NDEF’s payload of the NFC tag stuck in clothes.Finally,to evaluate the proposed method,it has been compared with default(digital)steganography weak points.Such as time,lockable,robustness,attacks,capacity,and a few more points.The results and comparisons showed that the proposed method is more efcient than default(digital)steganography and has many advantages. 展开更多
关键词 Near-eld communication radio-frequency identication STEGANOGRAPHY data hiding tags
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《塃老板》:十足真诚的矿山写真
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作者 王秋 《红河学院学报》 2007年第3期32-35,共4页
从叙述视点和情节、人物与态度两个方面对小说进行分析,探究文本在叙述过程以及人物塑造的过程中反映的态度和意图。叙述者对周家兄弟两人使用了双重价值判断标准,叙述者的知识分子口吻和故事内容也存在冲突,小说叙述有点断裂,影响了读... 从叙述视点和情节、人物与态度两个方面对小说进行分析,探究文本在叙述过程以及人物塑造的过程中反映的态度和意图。叙述者对周家兄弟两人使用了双重价值判断标准,叙述者的知识分子口吻和故事内容也存在冲突,小说叙述有点断裂,影响了读者心理自居的程度,在这个过程中却完成了对周老大和周老三的反讽,对传统道德观的质疑,具有了自省和批判的力度。 展开更多
关键词 叙述视点 反讽 戏仿 自居
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