Background： Adenomyosis （AM） has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells （MSMCs） of AM. Methods： Uterine ti...Background： Adenomyosis （AM） has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells （MSMCs） of AM. Methods： Uterine tissue samples from 22 patients （cases） with histologically confirmed AM and 12 （controls） with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calciumand voltage-sensitive K＋ channel （BKCa）-a/β subunits, voltage-gated potassium channel （Kv） 4.2, and Kv4.3. Student＇s t-test was used to compare the expression. Results： The BKCa-a/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-a messenger RNA （mRNA） （0.62 ± 0.19-fold decrease, P 〈 0.05） and Kv4.3 mRNA （0.67 ±0.20-fold decrease, P 〈 0.05） decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA. Conclusions： The BKCa-a mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.展开更多
The contraction of isolated rat and rabbit uteri induced by oxytocin and PGF2α was markedly inhibited by chlorpheniramine (Chl) and astemizolum (Ast) , both of which also decreased the resting tension of uteri, and t...The contraction of isolated rat and rabbit uteri induced by oxytocin and PGF2α was markedly inhibited by chlorpheniramine (Chl) and astemizolum (Ast) , both of which also decreased the resting tension of uteri, and their spontaneous contraction. The inhibitory effects of both drugs were dose-dependent. At high concentrations , Chl 7. 4 × 10-4 mol/L and Ast 10-4 mol/L could counteract the contraction of the uteri induced by Oxy and PGF2α, and their spontaneous contraction as well. They decreased the resting tension to the lower level. The mechanism of their non-special relaxed action on uteri could not be completely explained only by their H1-receptor blocking action. Whether they act by blocking calcium channel or by inhibiting calmodulin (CaM) remains to be further explored.展开更多
基金This study was supported by a grant of National Natural Science Foundation of China
文摘Background： Adenomyosis （AM） has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells （MSMCs） of AM. Methods： Uterine tissue samples from 22 patients （cases） with histologically confirmed AM and 12 （controls） with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calciumand voltage-sensitive K＋ channel （BKCa）-a/β subunits, voltage-gated potassium channel （Kv） 4.2, and Kv4.3. Student＇s t-test was used to compare the expression. Results： The BKCa-a/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-a messenger RNA （mRNA） （0.62 ± 0.19-fold decrease, P 〈 0.05） and Kv4.3 mRNA （0.67 ±0.20-fold decrease, P 〈 0.05） decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA. Conclusions： The BKCa-a mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.
文摘The contraction of isolated rat and rabbit uteri induced by oxytocin and PGF2α was markedly inhibited by chlorpheniramine (Chl) and astemizolum (Ast) , both of which also decreased the resting tension of uteri, and their spontaneous contraction. The inhibitory effects of both drugs were dose-dependent. At high concentrations , Chl 7. 4 × 10-4 mol/L and Ast 10-4 mol/L could counteract the contraction of the uteri induced by Oxy and PGF2α, and their spontaneous contraction as well. They decreased the resting tension to the lower level. The mechanism of their non-special relaxed action on uteri could not be completely explained only by their H1-receptor blocking action. Whether they act by blocking calcium channel or by inhibiting calmodulin (CaM) remains to be further explored.
