摘要
目的:探讨银杏叶中聚戊烯醇类化合物的分离纯化方法。方法:以聚戊烯醇的纯度为指标,筛选冷冻脱杂和硅胶柱层析最佳条件,获得含量90％以上的提取物。先用160倍量的石油醚-乙酸乙酯(9∶1)溶剂冷冻除杂,然后用硅胶柱层析进一步纯化(洗脱剂为石油醚-乙酸乙酯19∶1)。结果:精制后的聚戊烯醇纯度可达92.7％。结论:冷冻脱杂后再进行硅胶柱层析,可明显提高聚戊烯醇纯度,该方法重现性好,经济实惠,简单可靠。
Objective: To research the methods of purifying polyprenol from leaves of Ginkgo biloba L.. Methods: The purity of polyprenol Was determined by HPLC to select the optimal purifying conditions. Results: The optimal conditions were degreased by 160 times of petroleum ether-ethyl acetate(9:1) firstly, then through a silica gel column(100～140 mesh)and eluted with petroleum etherethyl acetate(19:1 ). Conclusion: The methods are feasible to purify polyprenol from leaves of Ginkgo biloba L. and could increase the purity of polyprenol obviously.
出处
《中药材》
CAS
CSCD
北大核心
2004年第5期337-339,共3页
Journal of Chinese Medicinal Materials
基金
江苏省教委自然科学基金(No.02KJD350002)
