摘要
目的:HPLC/外标法测定银杏叶中聚戊烯醇类化合物(PPAs,PPs)含量。方法:银杏叶样品10 g,加入石油醚100mL,室温下超声萃取45 min,共4次。提取物硅胶柱层析(硅胶100～140目,柱15 mm×350 mm),用石油醚-乙酸乙酯(90:10)洗脱。样品溶液采用HPLC分析:Inertsil ODS-3柱(4.6 mm×250 mm),柱温40℃,异丙醇-甲醇-正己烷-水(250:125:75:10)为流动相,流速1 mL·min^(-1),紫外检测器,检测波长215 nm。结果:聚戊烯醇类化合物中各组分基线分离,可同时分析银杏叶中PPAs、PPs含量,PPAs(或 PPs)进样量在0.26～5.2μg范围内与峰面积呈线性关系,PPAs、PPs平均加样回收率分别为98.8％和97.4％,RSD分别为1.8％和2.4％。结论:样品处理简单,结果稳定、准确,可作为银杏叶中聚戊烯醇类化合物的测定方法。
Objective: To determine polyprenols in Ginkgo biloba leaves using a reversed - phase HPLC/external standard method. Method: 10 g of leaf sample were ultrasound - extracted with 100 mL of petroleum ether four times for 45 min for each extraction at room temperature. The extract was subjected to a silica gel column (100-140 mesh, 15 mm × 350 mm) and eluted with petroleum ether - ethyl acetate (9: 1 ). The sample solution of PPAs (PPs) was analysed by using an Inertsil ODS - C18 column (5μm, 4. 6 mm×250 mm) at 40℃ with iso-prenol - methanol - hexane - water (250: 125 :75: 10) as mobile phase at a flow rate of 1 mL·min-1 . A UV detector was employed and the detection wavelength was set at 215 nm. Results: The polyprenol homologues were baseline separated and the determination of PPAs and PPs could be completed simultaneously. The calibration curve was linear in the range of 0. 26 -5.2μg. The average recoveries of PPAs and PPs were 98. 6% , 97. 4% with RSD of 1. 8% , 2. 4% respectively. Conclusion: The treatment of sample was simple and the analytical results were accurate. This method may be used for the determination of PPAs and PPs in Ginkgo biloba leaves.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第5期349-352,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金(29976018)
