摘要
目的：用高效液相色谱法测定中药莪术中３种姜黄素成分即姜黄素（ｃｕｒｃｕｍｉｎ，Ｉ）、脱甲氧基姜黄素（ｄｅｍｅｔｈｏｘｙｃｕｒｃｕｍｉｎ，ＩＩ）和双脱甲氧基姜黄素（ｂｉｓｄｅｍｅｔｈｏｘｙｃｕｒｃｕｍｉｎ，ＩＩ）。方法：采用ＩｎｅｒｔｓｉｌＯＤＳ３色谱柱，流动相为甲醇—异丙醇—水—冰乙酸（２０∶２７∶４８∶５），流速为０５ｍｌ·ｍｉｎ－１，检测波长为４２０ｎｍ。结果：３种姜黄素在０１～１９μｇ呈良好线性关系，方法回收率为９８２％～１０１１％，曾用本法测定了８种莪术样品。结论：本法准确，快速，重现性好，可用于考察研究中药莪术的不同品种以及同属其它中药的质量。
AIM: To determine curcuminoid constituents including curcumin, demethoxycurcumin and bisdemethoxycurcumin of rhizoma Curcumae. METHODS: A reversedphase HPLC system consisting of an ODS column and a mixture of methanol isopropanol water acetic acid (2027485) as the mobile phase was used. The flow rate was 05 mlmin-1 and detection was effected at 420 nm. RESULTS: The assay displayed good linearity over the concentration range of 0119 g and recoveries were 982%1011%. CONCLUSION: The method is accurate, rapid and reproducible and can be used for the quality study of rhizoma Curcumae.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第6期467-470,共4页
Acta Pharmaceutica Sinica
基金
国家九五攻关专项基金
关键词
姜黄素
高效液相色谱法
莪术
挥发油
curcumin
demethoxycurcumin
bisdemethoxycurcumin
rhizoma Curcumae
HPLC
