摘要
目的 :通过对决明子微波萃取法 (MAE)与常用提取方法 (索氏提取法、超声提取法、水煎法 )的比较研究 ,评价MAE提取中药有效成分的特点 ,初步探索MAE的机理。方法 :采用分光光度法测定决明子提取液中总蒽醌的含量 ;采用显微照相仪对表面结构及横切面状况进行观察。结果 :MAE的提取率最高 ,是超声提取法的 16倍 ,是索氏提取法的 3倍 ,是水煎法的 1.1倍 ,且MAE仅 5min就已超过超声 1h的提取率 ,15min已达到或接近索氏提取 2h和水煎法的提取效果 ;显微观察表明 ,微波直接造成表面结构的破坏。结论 :MAE用于中药决明子的提取具有高效、节能、省时的特点 ,可以在中药制药中进一步推广应用。
AIM: To evaluate the characteristic and explore the mechanism of MAE on Semen Cassiae by comparing MAE with commonly used extraction method. METHODS : The amount of anthraquinone was determined by spectrophotometer. The surface and cross section of Semen Cassiae were observed by microphotography. RESULTS : Among the four methods,the efficiency of MAE is 16 times that of ultrasonic extraction,3 times that of Sohlex extraction and 1.1 times of decocting by water,respectively. Micrographs taken after extraction differed markedly indicated that the degree of damage varied considerably. CONCLUSION : The MAE method is more advantageous than other traditional extraction methods (Soxhlet extraction and ultrasonic extraction) with regard to the extraction yield and the time and cost of the procedure.
出处
《中成药》
CAS
CSCD
北大核心
2004年第3期189-191,共3页
Chinese Traditional Patent Medicine
基金
清华大学化学工程国家联合重点实验室开放课题 (99 0 9)
关键词
微波萃取
决明子
总蒽醌
分光光度法
microwave-assisted extraction
Semen Cassiae
spectrophoto meter
spectrophotometer
