摘要
目的 :探讨淋巴细胞凋亡及凋亡蛋白表达的变化与特发性血小板减少性紫癜 (ITP)的关系。方法 :采用流式细胞仪检测 2 5例ITP患儿治疗前后外周血淋巴细胞凋亡率和Fas、FasL蛋白表达 ;采用酶联免疫夹心法 (ELISA)测定ITP患儿血清sFas、sFasL的水平。结果 :治疗后血小板恢复正常的患儿淋巴细胞凋亡率 (3 35± 1 4 3)、Fas(32 17± 6 5 4 )和FasL(41 14±6 76 )蛋白表达增加 ,与正常对照组 (0 85± 0 15、2 2 0 4± 4 5 4、2 0 83± 3 75 )和治疗前 (0 92± 0 17、2 3 0 3± 5 95、18 88± 4 5 7)相比差异有显著性意义 (P <0 0 0 1) ;治疗前ITP患者血清sFas含量为 15 5 4± 5 2 6 ,sFasL含量为 0 6 8± 0 2 3,均显著高于正常对照 (5 92± 1 78、0 33± 0 11,P <0 0 0 1)和治疗后组 (6 3± 1 92、0 36± 0 12 ,P <0 0 0 1)。结论 :ITP患者治疗后淋巴细胞凋亡增加可能与其治疗转归有关 ,sFas、sFasL的异常可能参与了ITP的免疫病理过程。
Objective:To investigate the relations between the changes of apoptotic lymphocytes and expression of apoptotic protein and idiopathic thrombocytopenic purpura(ITP).Methods:Flow cytometry was used to detect the percentages of the apoptotic lymphocytes and the expression of Fas,FasL on peripheral lymphocytes,and the serum levels of sFas、sFasL were also determined by a sandwich enzyme-linked immunosorbent assay(ELISA),in 25 patients with ITP.Results:The percentages of the apoptotic lymphocytes(3.35±1.43) and the expression of Fas(32.17±6.54) and FasL(41.14±6.76)were all increased significantly in treated patients whose platelets recovered to normal( P <0.001),as compared to normal controls(0.85±0.15、22.04±4.54、20.83±3.75)and untreated patients(0.92±0.17、23.05±5.95、18.88、4.57).The serum levels of sFas(15.54±5.26)、sFasL(0.68±0.23)in untreated patients were significantly higher than those of normal controls(5.92±1.78、0.33±0.11, P <0.001)and treated group(6.3±1.92、0.36±0.12, P <0.001).Conclusion:Incresed apoptosis of lymphocytes in treated patients may be related to the therapy and prognosis of ITP ,and the aberration of sFas、sFasL may be involved in the immunological pathogenesis of ITP.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第3期202-205,共4页
Chinese Journal of Immunology
基金
广东省卫生厅科研课题 (2 0 0 2 0 7)
