摘要
目的研究ITP患儿淋巴细胞亚群、血小板抗体及骨髓象在发病期的变化。方法应用流式细胞术(FCM)检测34例ITP患儿发病期外周血淋巴细胞亚群的水平(CD3+、CD4+、CD8+、CD19+、CD4+/CD8+、NK)。用ELISA法检测血小板表面血小板相关抗体IgG、IgM、IgA。同时抽取患儿骨髓制作骨髓片。结果ITP患儿发病期组CD4+/CD8+的比例显著降低,NK细胞显著降低,CD19+显著增高,ITP患儿组血小板抗体PAIgG、PAIgM和正常对照组比较显著增高(P<0.01),同时CD19+增高与血小抗体PAIgG、PAIgM增高有明显相关性(P<0.001)。骨髓象中巨核细胞总数和对照组比较显著增高(P<0.01),同时原幼巨核细胞总数、颗粒巨核细胞总数明显增高,而产板型巨核细胞总数明显降低。结论ITP患儿存在细胞免疫和体液免疫紊乱。
Objective： To investigate the changes of the diseases of lymphocyte subsets, PAIg, Bone marrow and effects on patho- logical mechanism of 1TP in the Children. Methods： The level of CD3 ＋ , CD3 ＋/CD4 ＋ , CD3 ＋/CD8 ＋ , CD19 ＋ , NK in 34 patients of Childrens diseases with ITP in the peripheral blood and PAIgG, PAIgA, PAIgM in platelets of 34 childrens diseases patients with ITP and 23 normal controls were measured by FACS and ELISA. At the same time , observed the childrens bone marrow by stain. Results ： Compared with the normal control, these children with ITP siginificantly decreaeed in percentage of CD3 ＋ cell, CD4 ＋ cell, NK cell and the ratio of CD4 ＋/CD8 ＋ cell but elevated in CD19 ＋ cell （ P 〈0.01） . The level of PAIgG, PAIgM in platelets were significantly higer than that in normal （ P 〈 0. 01 ） . The increasing percentage of CD19 ＋ cell were significantly corre- lated with the levels of PAIgG PAIgM in patients （ P 〈 0. 001 ） . The total of megakaryoblast, promegakaryocyto and granualar megakaryocyte were significantly increased but the total of thromocytogenic megakaryocyte were significantly decreased. Conclusion： There were disorder in celluar and humoral immunity of children with ITP.
出处
《中国优生与遗传杂志》
2008年第7期112-113,共2页
Chinese Journal of Birth Health & Heredity
