摘要
目的探讨急性特发性血小板减少性紫癜(AITP)患儿外周血淋巴细胞凋亡情况及凋亡蛋白Bc l-2和Bax蛋白表达情况。方法采AITP患儿外周血,分离淋巴细胞,用原位细胞凋亡方法检测淋巴细胞凋亡率,用免疫组化法检测淋巴细胞Bc l-2及Bax蛋白表达率。结果AITP患儿治疗前可见少量凋亡淋巴细胞,正常对照组未见明显凋亡细胞,Bc l-2蛋白表达率较正常对照组明显升高,Bax蛋白表达率较正常对照组降低。结论淋巴细胞凋亡异常在AITP发病机制中起重要作用。
Objective To investigate lymphocytes apoptosis , Bcl -2 protein and Bax protein expression in children with acute idiopathic thrombocytopenic purura （AITP）. Methods The blood samples were collected in children with AITP before treatment, in situ cell apoptosis （TUNEL method）, Bcl -2 protein and Bax protein expression （immunohistochemical method） were detected. Results There are a sprinkle of apoptotic lymphocytes in children with AITP before treatment, while there are few apoptotic lymphocytes in the controls. The rate of Bcl - 2 protein expression before treatment is increased,the rate of Bax protein expression is decreased. Conclusion The abnormal lymphocytes apoptosis in children with AITP might play an important role in its pathogenesis.
出处
《医药论坛杂志》
2006年第14期37-38,41,共3页
Journal of Medical Forum
