摘要
目的 ：观察清热凉血方、养阴退热方、补气方、活血方四类中药组方对特发性血小板减少性紫癜（ITP）模型小鼠脾脏Bcl-2、Fas表达的影响。方法 ：制备豚鼠抗小鼠血小板血清（APS）,采用腹腔注射APS方法,建立ITP小鼠模型。造模第5天开始各组灌胃给药治疗,正常组、模型组灌服生理盐水,清热凉血组、养阴退热组、补气组、活血组、醋酸沷尼松组小鼠分别灌服相应药物。连续给药10 d。用动物血液分析仪检测PLT,ELISA法检测脾淋巴细胞Bcl-2、Fas。结果 ：各造模组小鼠PLT显著降低,各造模组小鼠脾脏Bcl-2均明显升高,而Fas均明显降低,与正常组比较均差异显著（P〈0.001）。四类中药组方只有养阴清热组能明显降低小鼠脾脏Bcl-2,活血组明显升高小鼠脾脏Fas,与模型组比较差异显著（P〈0.05）。结论 ：养阴清热药及活血药对促进ITP模型小鼠脾脏淋巴细胞凋亡意义较大,为中医药治疗ITP有针对性选药提供一定实验依据。
Objective ：To observe the effects of traditional Chinese medicine prescriptions of Qingre Liangxue prescription,Yangyin Tuire prescription,Buqi prescription and Huoxue prescription on expression of platelet（PLT）,B-cell lymphoma-2（Bcl-2）and APO-1（Fas）in spleen of ITP model mice expression. Methods ：Guinea pig anti-mouse platelet serum（APS）was prepared by intraperitoneal injection of APS,and the ITP mouse model was established. The rats in the normal group and the model group were treated with normal saline,Qingre Liangxue group,Yangyin Tuire group,Buqi group,Huoxue group and acanthopanax senticosus group,respectively. Fed the corresponding drugs. For 10 days. PLT was detected by hematology analyzer,Bcl-2 and Fas was detected by ELISA. Results ：PLT of the mice in the model group was significantly decreased,Bcl-2 was significantly increased,Fas was significantly decreased. Compared with the normal group were significantly different（P〈0.001）. Yangyin Qingre group can obviously reduce Bcl-2 and Huoxue group can increase Fas. Compared with the model group,there was significant difference（P〈0.05）. Conclusion ：Yangyin Qingre medicine and Huoxue medicine have great significance for promoting the apoptosis of splenic lymphocytes in mice with ITP model,and provide some experimental basis for Chinese medicine to treat ITP.
出处
《辽宁中医药大学学报》
CAS
2018年第1期52-54,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
辽宁省教育厅一般项目(L2014366)
辽宁省自然科学基金项目(201602486)
关键词
四类中药组方
特发性血小板减少性紫癜
细胞凋亡
four traditional Chinese medicine prescriptions
idiopathic thrombocytopenic purpura
apoptosis
