摘要
本论文对滇型水稻品种玉优一号、HT - 7的愈伤组织诱导、基因转化及植株再生进行了研究。通过实验 ,将质粒 pCAMBI130 0上带有的PinⅡ抗虫基因导入水稻愈伤组织细胞中 ,获得了一批Hyg抗性植株 ,185株抗性苗经过PCR检测和叶片对潮霉素的抗性试验 ,证明其中有 5 9株为转基因阳性植株。结果显示 ,受体、农杆菌状态以及共培养方式等是影响基因转化效率的关键因素 ;以叶片对潮霉素的抗性作为转基因植株的快速筛选是可行的 ,大大提高了检测效率。
The material used in this experiment was Yunnan rice varieties Yuyou1 and HT-7.The calli induction, gene transformation and plant regeneration were carried out in the paper. The gene PinII in plasmid pCAMBI1300 was transferred into the cell of rice calli, some resistant plants were obtained by screened in hygromycin selecting culture.185 plantlets were used to be indentified by PCR and assayed leaf resistance to hygomycin, 59 plantlets of them were positive. The results showed that the status of receptor and Agrobacterium tumefaciens and co-culturing were the influencing factors in rice gene transformation, The Bio-assay of leaf resistance to hygomycin would be very effective in the selection of rice transgenic plants.
出处
《分子植物育种》
CAS
CSCD
2004年第1期19-24,共6页
Molecular Plant Breeding
关键词
农杆菌介导法
PinⅡ抗虫基因
滇型水稻
转基因
共培养
Yunnan rice (Oryza sativa L), Agrobacterium tumefaciens, Gene transformation, PinⅡgene, Co-culturing
