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基因枪法转基因水稻后代主要性状的多元遗传分析 被引量:1

Multivariate Genetic Analysis of Main Characters in Progenies of Transgenic Rice Mediated by Biolistic Bombardment
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摘要 对超级稻恢复系E 32及其20个基因枪法转基因T4代株系的生育期、纹枯病抗性及产量性状进行多元遗传统计分析。结果表明:12个性状主要载荷在5个主因子上,其相关信息占性状间总信息的92.44%。第一主因子中起主要作用的性状是穗总粒数、着粒密度和穗实粒数等粒数因子;第二主因子中载荷量较高的性状是病情指数、平均病级、病秆率等纹枯病抗性因子;第三主因子中起支配作用的性状是播始日数和生长日数等生育期因子;第四、五主因子中分别只有单株穗数、结实率起主导作用。根据斜交因子得分值把转基因后代株系分为4类,不同类型株系具有不同的生育期、抗病性和产量特征,育种中可依据转基因水稻各因子的相对重要性及各类群的特征,采取相应的育种改良措施以提高选择效率。 Characters of growth period, yield-related and resistance to sheath blight on T4 transgenic progenies of E 32 mediated by biolistic bombardment was investigated by multivariable heriditary analysis. The results indicated twelve indexes were divided into five common factors by factor analysis and the cumulative variance contribution of five factors reached 92.44%. Total grains per panicle( TGP), grains/cm( G/cm), filled grains per panicle(FGP) played important roles in the first factor. Disease index(DI), disease grade(DG) and disease stalk rate(DR) owned higher factor loading values in the second factor. The third factor was mainly dom- inated by days before heading(DH) and period of grow(DG). Only number of panicles(NP) and seed setting rate (SSR) played primary roles in the fourth factor and five factor respectively. Twenty transgenic progenies of E 32 werc divided into four types by clustering analysis according to the scores after promax rotation, varieties of different type had different characters of grow period, resistance to sheath blight and yield. Therefore, the improvement procedure can be an efficient way for rice breeding according to the relative importance of these principal factors and characters of different type varieties.
出处 《种子》 CSCD 北大核心 2007年第1期1-5,共5页 Seed
基金 国家"八六三"计划项目(101-01-02-02) 广东省"十五"重大科技专项项目(A2010101)
关键词 水稻 基因枪法 转基因 主要性状 因子分析 聚类分析 rice biolistic bombardment transgenic main characters factor analysis clustering analysis
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