摘要
采用基因枪法、电脉冲法、农杆菌介导法和浸泡法将外源基因质粒pZFX2导入小麦细胞中 ,经卡那霉素筛选 ,获得了抗性的愈伤组织和再生植株。通过此项试验 ,研究了不同方法的转化效果 。
Foreign plasmid pZFX2,containing the NPT Ⅱ(neomycin phosphotransferase)and glycolate oxidase gene which can increase the wheat plant resistance to insects,was introduced into the calli and mature embryos of wheat by the following methods:particle bombardment;electroporation,Agrobacterium mediated transformation and soaking in DNA solution.After selection culture and regeneration culture,some transgenic calli and plants resistant to Kanamycin were obtained.The optimum conditions and the efficiency of these gene transformation methods were studied.
出处
《西南农业学报》
CSCD
北大核心
2000年第4期25-29,共5页
Southwest China Journal of Agricultural Sciences
基金
云南省应用基础研究基金资助
关键词
小麦
基因转化
基因枪
电脉冲
农杆菌介导
wheat
gene transformation
particle bombardment
electroporation
Agrobacterium mediated transformation
plasmid pZFX2
soaking(
