摘要
采用体表接菌法测定了商品生物农药金龟子绿僵菌油悬浮剂PD20080671在15、20、25、30、35和40℃等6个不同温度条件下对东亚飞蝗3龄蝗蝻的致病力的影响。结果表明:东亚飞蝗3龄蝗蝻的累积死亡率在接菌后19 d内均随天数的增加而升高。35℃的累积死亡率最高(82.50%),显著高于其他处理;40℃的累积死亡率最低,为55.00%。死亡个体的湿度培养结果显示绿僵菌在高温条件下萌发的时间较短,40℃为4.26 d,15℃为19.36 d。结果表明环境温度低于15℃或高于35℃都不利于绿僵菌杀死蝗虫。因此,在田间施用金龟子绿僵菌防治东亚飞蝗时应尽量避免高温天气,建议在上午低温时进行防治。
The 3 rd instar nymphs of Locusta migratoria manilensis were treated by the commercial biopesticides Metarhizium anisopliae(PD20080671)through body surface inoculation under six different temperatures(15,20,25,30,35 and 40℃).The results showed that the cumulative mortality of locust nymphs increased within 19 d after inoculation.The highest(82.5%)was at 35℃and the lowest(55.0%)was at 40℃.Most of the dead locusts were 3 rd instars and a few were adults as the nyphms molted during the test period.Spore germination from of the dead locusts appeared sooner(4.26 d)at 40℃and took longer(19.36 days)at 15℃.The results indicate that temperature below 15℃and greater than 35℃are not optimal for fungal pathogenesis.These results demonstrate that the best time to apply biopesticides is early morning when the temperature is relatively low.
作者
刘银民
程雨蒙
李红梅
农向群
Belinda LUKE
LIU Yinmin;CHENG Yumeng;LI Hongmei;NONG Xiangqun;Belinda LUKE(MARA-CABI Joint Laboratory for Bio-safety/Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China;Academy of Life Science/Huaibei Normal University,Huaibei 235000,China;CABI East Asia,Beijing 100081,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China;CABI,Egham,TW209TY)
出处
《中国生物防治学报》
CSCD
北大核心
2019年第4期642-647,共6页
Chinese Journal of Biological Control
基金
国家重点研发计划(2017YFE0122400)
国家自然科学基金(61661136004)
关键词
商品生物农药
金龟子绿僵菌
东亚飞蝗
蝗蝻
biopesticides product
Metarhizium anisopliae
Locust migratoria manilensis
nymph
