摘要
从河南省汝阳县林地里采集到的天然罹病的黑蚱蝉僵虫中,分离到1株绿僵菌Metarhizium spp.,根据菌落培养特征和产孢结构特性,结合菌株ITS1-5.8s RNA-ITS2基因序列,鉴定为金龟子绿僵菌M.anisoliae,编号Ma121。测定了温度、光照、p H值以及碳氮源、微量元素对菌株Ma121菌丝生长和产孢的影响,得到菌株Ma121生长和产孢的适宜条件为温度25~30℃、全黑暗、培养基以大豆粉为碳源和蛋白胨为氮源并添加锰盐、p H 7。室内测定了菌株Ma121对东亚飞蝗若虫的致病力,结果显示菌株Ma121高浓度孢悬液(1×107~1×108孢子/m L)对若虫的校正累计死亡率均达到90%以上,致死中时(LT50)少于5 d。说明菌株Ma121能够有效侵染东亚飞蝗若虫并将其致死,菌株Ma121可以作为优良菌株进行杀蝗生物农药的进一步研发。
A strain of Metarhizium was isolated from a natural cadaver of black cicada in woodland, Ruyang County, Henan Province. The Metarhizium strain was identified as M. anisopliae and named Ma121 based on the gene sequence of ITS1-5.8s RNA-ITS2, morphological and cultural characteristics. The optimum culture conditions, including temperature, light, p H, carbon and nitrogen sources, microelement, for mycelia growth and sporulation of Mal21 were screened. The optimum conditions were determined to be temperatures at 25—30 ℃, p H 7, total darkness, soy powder and peptone as carbon and nitrogen source, respectively, with addition of Mn salt. Virulence of Ma121 to nymph of Locusta migratoria manilensis(oriental migratory locust) was evaluated in the laboratory. For spore suspension at a concentration of 1×107—1×108 spores/m L, the corrected accumulative mortality against the nymph was over 90%, with a LT50 less than 5 d. Therefore, the strain can be used as a potential biocontrol agent against the oriental migratory locust in the field.
出处
《中国生物防治学报》
CSCD
北大核心
2015年第3期333-339,共7页
Chinese Journal of Biological Control
基金
国家自然科学基金(31201558)
河南科技大学青年科学研究基金(13580012)
关键词
金龟子绿僵菌
东亚飞蝗
菌丝生长
产孢量
毒力测定
Metarhizium anisoliae
Locusta migratoria manilensis
mycelial growth
sporulation quantity
toxicity test
