摘要
为检测化学调控下甜叶菊叶片中不同甜菊糖苷的含量,建立了快速分析甜叶菊叶片中3种甜菊糖苷含量的高效液相色谱测定方法。液相色谱分析采用Sepax HP-Amino(4.6×250 mm,5μm)色谱柱,柱温40℃,流速1.00 mL/min,流动相采用等度洗脱模式,流动相乙腈/水组成比为80∶20(v/v),检测器为紫外-可见检测器,检测波长210 nm,谱带宽度20 nm,狭缝宽度16 nm,进样体积4μL;样品采用超声波辅助热水提取,澄清石灰水去除杂质。结果表明液相色谱分析得出3种甜菊糖苷(甜菊糖苷、莱鲍迪苷C、莱鲍迪苷A)线性方程分别为Y=1.075X-1.853(r=0.999 9)、Y=1.302 X+1.136(r=0.999 5)和Y=1.117X+1.089(r=0.999 8),在0.05～5.00 mg/mL之间线性关系均表现良好,方法检测限分别为1.091μg/mL,3.062μg/mL和1.078μg/mL。所建方法简单灵敏,适合叶片样品快速检测。
In order to determine steviol glycosides in stevia rebaudiana leaves grown by chemicals control,a rapid determination of three steviol glycosides in stevia rebaudiana leaves was developed by using high performance liquid chromatography. The HPLC analysis was performed on a Sepax HP-Amino column(4.6×250 mm,5 μm)by isometric elution using acetonitrile/water(80 ∶ 20,v/v)as the mobile phase. The column temperature was 40 ℃,and the ?ow rate was 1.00 mL/min. The wavelength of UV-vis detector was 210 nm. The width of spectrum band and spectral slit were 20 nm and 16 nm respectively. The injection volume was 4 μL. The steviol glycosides in samples were extracted with hot water by ultrasonic assisted method and the extracts were pretreated by clear limewater to remove impurities. The results showed that the calibration curves developed for three steviol glycosides(stevioside,rebaudioside C and rebaudioside A)were Y=1.075 X-1.853(r=0.999 9),Y=1.302 X+1.136(r=0.999 5)and Y=1.117 X+1.089(r=0.999 8)respectively,and all had a good linear relationship within the concentration range of0.05 ~ 5.00 mg/mL. The limit of detection were 1.091 μg/mL,3.062 μg/mL and 1.078 μg/mL,respectively. This method was simple and sensitive for detecting steviol glycoisdes in leave products.
作者
孙蕊
贾鹏禹
冯胜杰
冯乃杰
郑殿峰
SUN Rui;JIA Peng-yu;FENG Sheng-jie;FENG Nai-jie;ZHENG Dian-feng(Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《中国食品添加剂》
CAS
2019年第2期131-136,共6页
China Food Additives
基金
国家自然科学基金项目(No.31571613
31271652)
黑龙江省普通本科高等学校青年创新人才培养计划项目(No.UNPYSCT-2018086)
关键词
高效液相色谱
甜叶菊
叶片
甜菊糖苷
测定
high performance liquid chromatography
stevia rebaudiana
leaves
steviol glycosides
determination
