摘要
目的:建立高效液相色谱法测定复方鲜竹沥液(无糖型)中甜菊苷的含量。方法:选择CAPCELL PAK C18色谱柱(250 mm×4.60 mm,5μm),流动相为乙腈-水(33∶67),采用DAD检测器检测和采用外标法测定甜菊素中甜菊苷的含量,检测波长为210 nm。结果:甜菊苷峰面积与进样量间的线性关系良好,线性范围为0.2119~3.1781μg,平均回收率为99.43%(RSD=1.6%,n=6)。结论:该方法快速简便、结果准确、重现性好,可用于评价复方鲜竹沥液中药用辅料甜菊素的质量。
Objective:Establish an HPLC method for the determination of stevioside in fresh bamboo liquid(sugar free).Methods:The method used capcell Pak C18 column(250 mm×4.60 mm,5μm),acetonitrile water(33∶67)as mobile phase,Dad detector and external standard method to determine the content of stevioside in stevioside.The detection wavelength was 210 nm.Results:The sample volume and peak area of stevioside in fresh bamboo liquid compound(sugar free)had a good linear relationship.The linear range of stevioside was 0.2119-3.1781μg,the average recovery was 99.43%(RSD=1.6%,n=6).Conclusion:The method is quick,simple,accurate and reproducible,and can be used to evaluate the quality of steviolin in fresh bamboo liquid compound(sugar free).
作者
吴春花
李诒光
张民
胡子庆
杨凌宇
WU Chunhua;LI Yiguang;ZHANG Min;HU Ziqing;YANG Lingyu(Jiangzhong Pharmaceutical Co.,Ltd.,Nanchang Jiangxi 330000,China)
出处
《药品评价》
CAS
2021年第8期462-464,共3页
Drug Evaluation
关键词
甜菊苷
主成分分析
复方鲜竹沥液
高效液相色谱法
含量测定
Stevia
Principal component analysis
Fresh bamboo liquid compound
HPLC
Content determination
