摘要
目的 探讨喉鳞状细胞癌组织中转移相关基因 (metastasis associatedgene1 ,MTA1 )的表达与颈淋巴结转移的关系。方法 应用逆转录 聚合酶链反应 (reversetranscriptionpolymerasechainreaction ,RT PCR) ,检测 32例声门上型喉鳞状细胞癌 (1 6例伴颈淋巴结转移、1 6例不伴颈淋巴结转移 )、1 6例声门型喉鳞状细胞癌 (均不伴颈淋巴结转移 )、1 6例喉正常组织中MTA1mRNA的表达。结果 RT PCR结果显示在 1 6例声门上癌颈淋巴结转移组的癌组织中 ,MTA1mRNA表达阳性 ;在不伴颈淋巴结转移的其他喉鳞状细胞癌组织 (声门上型 1 6例和声门型 1 6例 )及 1 6例喉正常组织中MTA1mRNA表达阴性。结论 MTA1mRNA的表达与喉鳞状细胞癌颈淋巴结转移密切相关 。
Objective The purpose of study was to examine the mRNA expression levels of the metastasis associated gene1 (MTA1) in laryngeal squamous cell carcinoma(LSCC), so to evaluate its relationship with metastases Methods Fourty eight surgically resected primary LSCC(16 supraglotti laryngeal carcinomas with cervical lymph node metastasis,16 supraglotti laryngeal carcinomas with cervical lymph node negative metastasis,16 glottic carcinoma with cervical lymph node negative metastasis )and 16 normal laryngeal mucosa was examined for mRNA expression of MTA1 by reverse transcription polymerase chain reaction(RT PCR) Results The frequency of MTA1 mRNA positive expression in 16 LSCC with cervical lymph node metastasis was 100% ,but no expression of MTA1 mRNA was observed in other LSCC with cervical lymph node negative metastasis (16 supraglotti laryngeal carcinomas and 16 glottic carcinoma) and 16 normal laryngeal mucosa Conclusion MTA1 gene is strongly related to cervical lymph node metastasis of LSCC,and may serve as a early diagnosis indicator
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2003年第3期213-216,共4页
Chinese Journal of Otorhinolaryngology
