摘要
目的 :分析原发性前列腺癌及高级别前列腺上皮内肿瘤 (PIN) 8号染色体等位基因杂合性缺失 (LOH)并探讨其意义。 方法 :经显微切割获取前列腺癌及PIN各 10个样本DNA ,采用PCR及微卫星多态性技术 ,对 8号染色体上的 14个微卫星位点LOH进行检测。 结果 :10个原发性前列腺癌样本在 8号染色体有不同LOH的频率 ,8p 2 3.1 p2 3.2及 8p2 1 p2 2为两个高频LOH区。 10个高级别PIN样本检测 8号染色体 14个微卫星位点 ,有 5个样本至少有一个位点检测到LOH。 结论 :前列腺癌中存在 8号染色体的高频LOH区 ,分别位于 8p 2 3.1 p 2 3.2 ,8p2 1 p2 2区 ,高级别PIN出现LOH的位点与前列腺癌相同 ,位于 8p 2 3.1 p 2 3.2及 8p2 1 p2
Objective: To detect the status of loss of heterozygosity (LOH) on chromosome 8 in prostate carcinoma and high grade prostatic intraepithelial neoplasia (PIN). Methods: Pure DNA was obtained from prostate neoplasms and normal tissues by tissue microdissection. LOH on chromosome 8 was detected by PCR based microsatellite polymorphism analysis technique using 14 pairs of microsatellite primers in 10 samples of prostate carcinoma and 10 samples of high grade PIN. Results: There were different frequencies of LOH on chromosome 8 in 10 samples of prostate carcinoma. 8p 23.1-p 23.2 and 8p21-p22 were two high-frequency LOH regions. LOH on chromosome 8 was detected in 3 samples of high grade PIN. Conclusions: There were high-frequency LOH regions on chromosome 8 of prostate carcinoma, located on 8p 23.1-p 23.2 and 8p21-p22. High grade PIN and prostate carcinoma share the same allelic loss on 8p.Tumor suppressor genes located at these two regions may be potentially involved in the initiation and progression of prostate carcinoma.
出处
《中华男科学杂志》
CAS
CSCD
2004年第1期26-28,31,共4页
National Journal of Andrology
