摘要
目的 检测原发性前列腺癌及高级别前列腺上皮内瘤 (PIN) 6号染色体等位基因杂合子丢失 (LOH)及其意义。方法 经显微切割技术获取前列腺癌及PIN各 10例患者DNA ,采用聚合酶链反应 (PCR)及微卫星多态性技术 ,对 6号染色体上的 2 0个微卫星标志位点LOH进行检测。结果 10例原发性前列腺癌中有 8例在 6号染色体上至少有 1个位点检测到LOH ,6q2 1 6q2 3及 6q2 5 6q2 7为2个高频LOH区。 10例高级别PIN检测 6号染色体 2 0个位点 ,有 5例各有 1个位点检测到LOH。结论 前列腺癌中存在 6号染色体的高频LOH区 ,分别位于 6q2 1 6q2 3、6q2 5 6q2 7区 ,编码细胞周期素C及胰岛素样生长因子Ⅱ受体的基因为此 2区候选的抑癌基因 ,它们可能与前列腺癌的发生发展有关。
Objective To detect the significance of loss of heterozygosity (LOH) on chromosome 6 in prostate carcinoma and high grade prostatic intraepithelial neoplasia (PIN). Methods Pure DNA was obtained from prostate neoplasms and normal tissues after tissue microdissection. LOH of chromosome 6 was detected by PCR based microsatellite polymorphism analysis technique using 20 pairs of microsatellite primers in 10 prostate carcinoma cases and 10 high grade PIN cases. Results Allelic loss at one or more loci was observed on chromosome 6 in 8 of 10 prostate carcinoma cases. 6q21-6q23 and 6q25-6q27 were two of LOH high frequency regions. 5 high grade PIN cases had LOH detected on chromosome 6. Conclusions Two high frequency LOH regions were detected on chromosome 6 of prostate carcinoma. Cyclin C, IGF2R genes were two candidate tumor suppressor genes located in these two regions, they may be involved in the initiation and progression of prostate cancer.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2001年第6期414-417,共4页
Chinese Journal of Pathology
关键词
前列腺肿瘤
前列腺上皮内瘤病
杂合子丢失
染色体
人
6对
Prostatic neoplasms
Prostatic intraepithelial neoplasia
Loss of heterozygosity
Chromosomes, human, pair 6
