摘要
目的:对T细胞淋巴瘤(T-cell lymphoma,TCL)6号染色体上6个微卫星多态标志物进行等位基因杂合性缺失(loss of heterozygosity,LOH)分析,以明确该区域是否存在与人类TCL发生发展相关的抑癌基因。方法:选取6号染色体上6个微卫星多态标志D6S251、D6S275、D6S287、D6S267、D6S262、D6S264,采用石蜡组织基因组DNA抽提、PCR扩增,变性聚丙烯酰胺凝胶垂直电泳、银染法分别检测了42例TCL中肿瘤组织与相应正常组织基因组DNA的LOH状况。结果:42例TCL中13例(13/42,30.95%)至少在1个位点出现LOH,以D6D262最高(10.3%),其次为D6S287(10.0%)和D6S267(7.3%)。而不同临床病理分型的TCL其LOH发生差异无统计学意义(P>0.05)。结论:在6号染色体上的6个微卫星标志中D6S287、D6S262和D6S267周围的6q21-6q23压域发生杂合性缺失率较高,位于6q21区编码Cyclin C的基因可能是此区与TCL发生发展相关的候选抑癌基因;尤其是6q21-6q22.1区域可能存在与TCL相关的抑癌基因,可能与TCL的发生发展有关。
Objective：To study the loss of heterozygosity （LOH） at 6 markers of microsatellite polymorphism on the chromosome 6 in T-cell lymphoma （TCL） to determine whether there existed tumor suppressor genes in the area related with the initiation and development of TCL. Methods：Six microsatellite polymorphism markers （D6S251, D6S275, D6S287, D6S267, D6S262, and D6S264） on the chromosome 6 were selected. We performed the amplification of microsatellite DNA with PCR, polyacrylamide gel electrophoresis and silver staining to detect LOH in 42 cases of T-cell lymphoma and the corresponding normal tissues. Results：LOH were detected at more than one locus in 13 out of 42 TCL patients （30.95%）. Among the 6 loci, LOH occurred more frequently at D6S262 （ 10.3% ）, D6S287 （10.0%）, and D6S267 （7.3%）. No significant difference was found in LOH incidence between different clinicopathologic classifications （P 〉0.05）. Conclusion：The LOHs occur more frequently at markers D6S262, D6S287, and D6S267 on the chromosome 6q21 to 6q23. Cyclin C gene localized to chromosome 6q21 may be the candidate of tumor suppressor gene related with initiation and progression of TCL. Chromosome 6q21-6q23 may harbor a tumor suppressor locus which was related with TCL.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第9期683-686,共4页
Tumor
基金
福建省卫生厅青年科研课题资助计划项目(编号:2005-2-24)
