摘要
目的选择食管癌中遍布10个染色体区域的17个微卫星位标，进行食管癌手术切除标本的微卫星杂合缺失（LOH）分析，并探讨这些位点的LOH与患者临床病理参数之间的关系。方法68份食管癌患者手术切除的鳞癌组织标本中，高分化鳞癌20份，中分化鳞癌30份，低分化鳞癌18份。采用PCR荧光测序法和凝胶电泳分别检测68例患者的鳞癌组织及匹配的外周血样本中17个微卫星位点的杂合性缺失状况；并比较分析LOH与肿瘤临床病理参数之间的关系。结果68份标本中检出位点D8S261 LOH频率最低（33．3％），检出位点D9S125 LOH频率最高（85．2％），其中，12个位标LOH频率高于50．0％，3个（D3S1597、D3S1285、D9S125）高于75．0％；分化程度不一的肿瘤标本在位点D9S111与D13S153处的LOH频率差异有统计学意义（Х^2=15．5、10．8，P均〈0．05），位点D9S111处高分化的12份标本中发生缺失2份，中分化20份标本中发生缺失14份，低分化16份标本中发生缺失14份，位点D13S153处8份高分化标本中2份发生缺失，中分化的28份标本中12份发生缺失，低分化的12份标本中11份发生缺失；发生淋巴结转移的肿瘤标本与未转移标本在位点D8S261处的LOH频率差异有统计学意义（Х^2=6．4，P〈0．05），位点D8S261处发生转移的14份标本中1份出现缺失，未转移的22份标本中12份出现缺失。结论食管鳞癌中存在广泛、高频的LOH，位于高频缺失位点附近的基因可能参与了食管癌的发生发展；位点D9S111和D13S153处的缺失与食管癌的病理分级有显著的相关性，分化越差的标本在2位点处缺失频率倾向越高；位点D8S261处的缺失与食管癌淋巴结转移呈反向相关，在该处发生缺失的标本淋巴结转移的可能性往往较低。
Objective To investigate the loss of heterozygosity at 17 microsatellites of 10 chromosome arms in 68 resected specimens of esophageal cancer, and the relationship to the clinicopathological phenotypes of patients. Methods 68 tumor specimens （20 well-differentiated squamous carcinomas, 30 moderately differentiated carcinomas and 18 poorly differentiated carcinomas） and their matched blood samples were analyzed for LOH at 17 mierosatellites by using PCR and fluorescence-based DNA sequencing technology, and the association of LOH with the clinicopathological phenotypes of patients was compared statistically. Results The lowest detection frequency of LOH in our subjects was observed at D8S261 with 33.3% , and the highest frequency was at D9S125 with 85.2%. There were 12 markers with the frequency of LOH higher than 50.0% , and 3 markers （ D3S1597, D3S1285 and D9S125） with the frequency higher than 75.0%. There was a significant difference in the frequency of LOH at D9S111 and D13S153 between tumors with different histological grades. LOH at D9S111 was observed in 2 of 12 tumors with well differentiation in 14 of 20 tumors with moderate differentiation, and in 14 of 16 tumors with poor differentiation. LOH at D13S153 was observed in 2 of 8 tumors with well differentiation, in 12 of 28 tumors with moderate differentiation, and in 11 of 12 tumors with poor differentiation. There was a significant difference in the frequency of LOH at D8S261 between tumors with lymph node metastasis and without lymph node metastasis. LOH at D8S261 was found in 1 of 14 tumors with lymph node metastasis, and in 12 of 22 tumors without lymph node metastasis. Conclusions The widespread and frequent loss of heterozygosity may exist in esophageal cancer, and the candidate genes located in the site of frequent LOH may be involved in the development of this cancer; LOH at D9S111 and D13S153 are more commonly observed in the patients with higher histological grades, the tumors with LOH at D8S261 may have a low tendency to lymph node invo
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2009年第7期810-813,共4页
Chinese Journal of Laboratory Medicine
关键词
食管肿瘤
微卫星重复
杂合子丢失
Esophageal neoplasms
Microsatellite repeats
Loss of heterozygosity
