摘要
目的 探讨原发性前列腺癌及高级别前列腺上皮内肿瘤 (PIN ) 2 2条常染色体等位基因杂合性缺失及其意义。方法采用显微切割技术提取前列腺癌及高级别前列腺上皮内肿瘤各 10例的DNA ,然后采用PCR及微卫星多态性技术 ,对 2 2条常染色体上的 382个微卫星标志位点进行高密度allelotype分析。 结果 10例原发性前列腺癌在染色体 1p、1q、2 p、2 q、6q、8p、8q、10q、11q、13q、16p、17p等部位存在高频杂合性缺失 (LOH ) ,前列腺上皮内肿瘤则在染色体 2 p、2q、6q、8p、10q、1q等区易检测到LOH。结论前列腺癌患者 2 q14、8p12～ 2 1、10 q2 3～ 2 4、13q14等区域存在高频的LOH ,位于这些区域的抑癌基因在前列腺癌发生。
Objective To detect the status of loss of heterozygosity (LOH) on 22 autosomes in human primary prostate carcinoma and high grade prostatic intraepithelial neoplasia(PIN).Methods Pure DNA was obtained from prostatic neoplasms and normal tissues by tissue microdissection.LOH of 22 autosomes were detected by PCR based high resolution allelotype analysis technique using 382 pairs of microsatellite primers in 10 cases of prostatic carcinoma and 10 cases of high grade PIN.Results There were high frequency of LOH on 1p,1q,2p,2q,6q,8p,8q,10q,11q,13q,16p,17p in prostate carcinomas.LOH had also been detected on chromosome 2p,2q,6p,8q,10q,11q in high grade PIN.Conclusion Frequent deletion on chromosome 2q14,8p12～21,10q23～24,13q14 in human prostate neoplasms suggests the presence of tumor suppressor genes on these regions that potentially be involved in the initiation and progression of prostate cancer.
出处
《实用癌症杂志》
2002年第2期148-150,共3页
The Practical Journal of Cancer
