摘要
目的检测宫颈鳞状细胞癌组织和上皮内瘤变组织中分化抑制因子(Inhibitors of differentiation 1,Id-1)和p53的表达并分析其相关性,与非肿瘤宫颈组织做对照,探讨二者在宫颈鳞状上皮癌变过程中的作用和临床意义。方法采用免疫组化(SP)二步法检测宫颈鳞癌(cervical squamous cell carcinoma,SCC)64例(其中高分化15例,中分化18例,低分化31例,FIGO分期Ia^IIa期)、宫颈上皮内瘤变(cervical intrae Pithelial neoPlasia,CIN)34例(其中CINI级4例,CINII级14例,CINIII级16例)及对照组30例(即正常宫颈鳞状上皮组织)中Id-1与p53蛋白的表达情况,分析Id-1表达水平与宫颈鳞癌临床病理特征间的关系及与p53表达水平的相关性。结果对照组、CINI组、CINⅡ组、CINⅢ组及SCC组中Id-1的阳性表达率分别为0%、25.0%、50.0%、56.3%、73.4%;p53表达率分别为0%、25.0%、50%、62.5%、71.9%;宫颈鳞癌组织分化级别越低和有盆腔淋巴结转移者Id-1表达阳性率越高,Id-1和p53在对照组中的表达与CIN组及SCC组相比较,差异均有统计学意义(P<0.05)。在宫颈鳞状上皮癌变过程中中Id-1和p53表达水平呈现正相关(rs=0.631,P<0.01)。结论随着宫颈鳞状上皮癌变过程的进展,Id-1和p53的表达逐渐增强,Id-1和p53的联合检测可作为宫颈癌前病变分级、宫颈鳞癌病理诊断的参考指标。
Objective To explore the significance and relationship of Id- 1 and p53 in development and progress of cervical squamous cell carcinoma. Methods The tissues for detecting expression of Id- 1 and p53 proteins came from 34 patients suffered CIN( including CIN I 4 cases,CIN II 14 cases,CIN III 16 cases),64 patients with SCC( SCC group) and 30 cases of normal cervical epithelia( control group) and the expression were examined by immunohistochemical staining( SP). The relationship between the expression outcomes of Id- 1 and the clinical pathological characteristics of cervical cancer,and the expression outcomes of p53 were analyzed. Results The positive rate for Id- 1 expression in the control group,the CIN I group,the CIN II group,the CIN III group and the SCC group was 0%,25. 00%,50. 00%,56. 25% and 73. 44%,respectively and the positive rate for p53 expression was 0%,25. 0 %,57. 14%,68. 75% and 71. 87%,respectively. The expressions of Id- 1 and p53 in the SCC group and the CIN groups were significantly higher than those in the control group( P < 0. 05). Id- 1 expression was positively correlated with p53 expression in the process of carcinogenesis of cervical squamous cell carcinoma( rs= 0. 631,P < 0. 01). Conclusion With the development and progress of cervical squamous cell carcinoma,Id- 1 and p53 expression gradually increased,indicating that the two factors were jointly participated in the pathogenesis and development of cervical squamous cell carcinoma. Detection of Id- 1 combined with p53 might be extremely helpful for the diagnosis of CIN and cervical SCC.
出处
《宁夏医科大学学报》
2015年第9期1022-1025,封4,共5页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ13173)
关键词
宫颈鳞癌
ID-1
P53
免疫组化
cervical squamous cancer
Id-1
p53
immunohistochemical staining
