摘要
目的探讨细胞分化抑制因子-1(Id-1)在宫颈癌、宫颈上皮内瘤变(CIN)及正常宫颈黏膜组织中的表达及临床意义,并评价其与细胞增殖核抗原Ki-67的相关性。方法收集60例宫颈癌、60例CIN及30例正常宫颈黏膜组织,用链霉素亲和生物素-过氧化物酶免疫组化法检测Id-1和Ki-67的表达并进行比较;同时,分析Id-1及Ki-67在宫颈癌中表达的相关性及两者与宫颈癌病理参数的关系。结果 Id-1在正常宫颈黏膜组织、CIN低度病变组织、CIN高度病变组织及宫颈癌组织中的阳性表达率分别为6.67%,10.81%,56.52%,76.67%,差异有统计学意义(P<0.05);Ki-67在正常宫颈黏膜组织、CIN低度病变组织、CIN高度病变组织及宫颈癌组织中的阳性表达率分别为10.00%,21.62%,65.21%,80.00%,差异有统计学意义(P<0.05)。Id-1和Ki-67的表达与宫颈癌的分化程度、淋巴结转移、脉管浸润及宫颈癌国际妇产科联盟分期呈正相关(P<0.05)。结论 Id-1表达上调可能与宫颈癌的发生发展有一定的关系,Id-1、Ki-67蛋白的表达在宫颈癌的发生发展中可能存在一定的协同作用。
Objective To investigate the expression of inhibitor of differentiafion-1（Id-1） in tissues of cervical cancer,cervical intraepithelial neoplasma（CIN） and normal cervical tissue,and its correlation with Ki-67.Methods The expression of Id-1 were examined in cervical cancer（60 cases）,CIN（37 cases of CIN Ⅰ and 23 cases of CINⅡ-Ⅲ）,and normal cervical tissues by immunohistochemical SP method.The expression of Ki-67 in 60 cervical cancer tissue were detected and the correlation between the expression of Id-1 and Ki-67 protein in cervical carcinoma were analyzed.Results The positive rates of Id-1 were 6.67%,10.81%,56.52% and 76.67% in normal tissue,CIN Ⅰ,CIN Ⅱ-Ⅲ and cervical cancer tissues,respectively（P〈0.05）,and 10.00%,21.62%,65.21%,80.00% in terms of the positive rate of Ki-67.The expressions of Id-1 and Ki-67 were positively correlated with the degree of cancer differentiation,vascular invasion,lymph node metastasis,and International Federation of Gynecology and Obstetrics（FIGO） stage（P〈0.05）.Conclusion Up-regulation of Id-1 expression may have a certain relationship with the occurrence and development of cervical cancer,and the expression of Id-1 and Ki-67 has synergistic effect on the occurrence and development of cervical cancer.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第8期627-630,共4页
The Chinese Journal of Clinical Pharmacology
关键词
宫颈癌
细胞分化抑制因子-1(Id-1)
细胞增殖核抗原Ki-67
免疫组化法
cervical cancer
inhibitor of differentiafion-1（Id-1）
cellular proliferative nuclear antigen Ki-67
immunohistochemical streptavidin-perosidase technique
