摘要
利用不同激素组合对苦瓜愈伤组织诱导、芽的分化及试管苗快速繁殖进行了研究.结果发现不同外植体均易诱导愈伤组织,但愈伤组织芽分化较难,而外植体在一些激素组合中可直接形成不定芽;利用无菌苗的顶芽及腋芽,培养于附加6 BA1mg L+NAA0.5mg L或NAA1mg L的MS培养基上,获得了芽的快速繁殖,形成芽簇,每丛芽数达6～8苗;试管苗于1 2MS+NAA0.5mg L生根培养基中6d生根率达到100%,获得完整再生植株.结果表明采用不同激素组合对苦瓜体细胞形态发生与发育的调控有决定性作用.
The research investigated hormone control on calli induction, differentiation, and propagation of Momordicacharantia by means of tissue culture. The results found that various explants are easy to induce calli but difficult to differentiate into bud. The bud explants were cultured on MS media supplemented with 1 mg/L 6-BA and 0.5 ∼ 1 mg/L NAA, which stimulated proliferation of the bud meristrems to form bud clusters, the co-efficient reached 6 ∼ 8; the buds transferred to MS media supplemented with 1 mg/L 6-BA grow healthily and turn into rootless plantlets. On media 1/2 MS containing 0.5 mg/L NAA, the plantlets begin to root in 6 days, the frequency of rootage can reach 100%. The results help to clarify the effects of hormone's influence on morphog etnisis and development of Momordicacharantia.
出处
《湖南师范大学自然科学学报》
EI
CAS
北大核心
2003年第4期76-78,共3页
Journal of Natural Science of Hunan Normal University
