摘要
目的探讨电针对卵巢储备功能下降(DOR)患者体外受精-胚胎移植(IVF-ET)中间结局指标的影响。方法采用前瞻性、自身对照研究方法对37例DOR患者进行观察。所有患者在促排卵治疗前均接受电针治疗,每周治疗3次,总疗程为3个月经周期;在电针结束后进入IVF-ET周期。测定治疗前后血清性激素、抗苗勒氏管激素(AMH)、促排卵相关参数、获卵数、胚胎发育情况。结果患者AMH水平较治疗前显著升(P<0.05);而bFSH、bFSH/LH、bE_2、P、HCG日E_2及P水平无明显变化(P>0.05)。患者基础窦卵泡(bAFC)、获卵数较干预前增加,但差异无统计学意义(P>0.05);而成熟II级卵子(MII)数、受精数、优质胚胎数较干预前均显著增加,差异有统计学意义(P<0.05)。结论电针疗法可以提高卵巢储备功能,促进卵泡的生长、发育,其对促排结局的改善可能是建立在对AMH的良性调节基础上的,但其作用机制还有待于今后进一步的研究。
Objective To observe the effect of electroacupuncture upon intermediate outcome indicators for diminished ovarian reserve(DOR)patients who plan to conduct in vitro fertilization-embryo transfer(IVF-ET).Methods A prospective,self-controlled study was carried out on 37 DOR patients.Electroacupuncture treatment was given to all the patients three times a week for 3 consecutive menstrual cycles before ovulation induction.Upon that,the patients were treated with IVF-ET.Serum sex hormones,anti-Mullerian hormone(AMH)ovulation induction parameters,numbers of ovum and embryo development were measured before and after the treatment.Results The level of AMH in patients was significantly higher after treatment(P<0.05),however,there was no significant change in basic serum follicle-stimulating hormone(bFSH),follicle-stimulating hormone/luteinizing hormone ratio(bFSH/LH),estradiol(E2),progesterone(P),E2 and P levels on the day of HCG(P>0.05).Basal sinus follicle count(bAFC)and the numbers of acquired eggs increased,but the difference was not statistically significant(P>0.05).The numbers of II mature oocytes,fertilization and high-quality embryos increased significantly compared with those before the intervention(P<0.05).Conclusion Electroacupuncture can improve ovarian reserve function and promote the growth and development of follicles,and its improvement on the outcome of induced ovulation may be based on the benign regulation of AMH,but the mechanism remains to be further studied in the future.
作者
高友玲
沈洁
陈莉
穆艳云
陈欢
席瑾
何婷
夏有兵
GAO You-ling;SHEN Jie;CHEN Li;MU Yan-yun;CHEN Huan;XI Jin;HE Ting;XIA You-bing(Nanjing University of Chinese Medicine,Nanjing 210023,China;Yangzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine,Yangzhou 225600,China;Nanjing General Hospital of Nanjing Military Region,PLA,Nanjing 210002,China;Jiangsu Province People’s Hospital,Nanjing 210029 China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2019年第2期380-382,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(81473767
81804179)
江苏省中医药局科技项目(YB2015005
YB2017005)
朱新太江苏省名老中医药专家传承工作室
关键词
电针
卵巢储备功能下降
体外受精-胚胎移植
抗苗勒氏管激素
Electroacupuncture
Diminished ovarian reserve(DOR)
In vitro fertilization-embryo transfer(IVF-ET)
Anti-Mullerian hormone(AMH)
