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粮食及果蔬中叶酸含量分析 被引量:20

Analysis of Folic acid Contents in Main Grain Crops, Fruits and Vegetables
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摘要 对日常食用的主要粮食及果蔬的叶酸含量进行测定与评价,为人体科学合理补充叶酸提供理论依据。在山西省各农户及市场搜集11种主要粮食、17种常见蔬菜、8种常见水果。粮食样品研磨全部过100目筛子备用,市售新鲜果蔬样品将可食用部分随机多点采样,切碎混合。食物叶酸用磷酸二氢钾溶液恒温水浴浸提,加苯胺处理过的活性炭吸附,用体积分数3%氨-70%乙醇洗脱,采用高锰酸钾氧化-间接荧光法测定。结果显示,主要食物叶酸含量存在差异,叶酸含量较高的粮食种类有:大豆、花生、绿豆、小米、燕麦、玉米和苦荞,含量均在1.56μg/g(以干质量计)以上;含量较高的蔬菜有:菠菜、油菜、香菇、油麦菜、生菜、卷心菜、青椒、芹菜、白菜和南瓜,含量均在1.53 μg/g(以湿质量计)以上;人们日常食用的水果叶酸含量均在1.74μg/g(以湿质量计)以上;只要合理调整膳食结构,粗细搭配,多摄食蔬菜水果,就有望通过食疗来补充人体所缺乏的叶酸。 In this study, the contents of folic acid in main grain crops, fruits and vegetables were determined and evaluated to provide a theoretical basis for reasonable supplementation of folic acid for people's daily consumption. A total of 38 samples, including 11 kinds of main grain crops, 17 kinds of common vegetables and 8 kinds of common fruits, were collected from some households and local markets in Shanxi Province. Grain samples were prepared by grinding and sieving through 100 mm sieve, and fresh fruits and vegetables samples were randomly prepared by collecting the edible parts and were chopped into small pieces. The contents of folic acid in all samples were extracted with potassium dihydrogen phosphate in water bath. The optimum extraction was achieved by addition of the active carbon adsorbent treated by aniline to the extraction solvent, the extracts were eluted continuously with a mixture containing 3% ammonia and 70%ethanol, and indirect fluorescent method was used to determine the fluorescent intensity of folic acid oxidized by potassium permanganate. Data showed that there was a significant difference in the contents of folic acid between the main grain crops, which were all over 1.56 μg/g md, and the grain crops containing higher folic acids included soybean, peanut, mung bean, foxtail millet, oat, corn and buckwheat. The vegetables with higher folic acid content surpassing 1.53 μg/g mf were in decreasing order: spinach, rapeseed, shiitake, leaf lettuce, lettuce, cabbage, green pepper, celery, Chinese cabbage and pumpkin. The folic acid content in the fruits for daily consumption was over 1.74 μg/g mf. These data may provide a scientific basis for reasonable dietary supplementation of folic acid as well as reasonable adjustment in dietary structure based on intake of fruits and vegetables.
作者 邵丽华 王莉
出处 《食品科学》 EI CAS CSCD 北大核心 2014年第24期290-294,共5页 Food Science
基金 2012年度校级大学生创新性实验项目(SD2012CXSY-31) 山西省引进优秀人才专项(2010-37) 山西省回国留学人员科研资助项目(2011-056)
关键词 粮食 水果 蔬菜 叶酸含量 grain crop fruit vegetable folic acid content
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