摘要
目的建立黄花柳花中5个黄酮类成分含量测定的一测多评方法。方法采用高效液相色谱法,以芦丁为参照对照品,计算其与木犀草素-7-О-β-D-吡喃葡萄糖苷、芹菜素-7-O-β-D-吡喃葡萄糖苷、木犀草素、芹菜素的相对校正因子,考察相关影响因素并确立相对校正因子。样品测定中以芦丁含量按相对校正因子直接计算出其他4个成分的含量,从而建立起以芦丁为对照品的一测多评法,并与外标法测得值比较验证。结果相对校正因子分别为f_(木犀草素-7-O-β-D-吡喃葡萄糖苷/芦丁)=1.49(RSD=1.84%)、f_(芹菜素-7-O-β-D-吡喃葡萄糖苷/芦丁)=2.26(RSD=2.26%)、f_(木犀草素/芦丁)=2.63(RSD=1.01%)、f_(芹菜素/芦丁)=2.86(RSD=1.55%),重复性良好。通过t检验,样品的一测多评法计算值与外标法测得值之间无显著性差异,实验所得的校正因子可信。结论建立的一测多评法同步测定黄花柳花中5个黄酮类成分的含量是可行的、准确的,可用于黄花柳花的质量评价。
Objective To establish the method of quantitative analysis of multi-components by single-marker(QAMS) in the determination of five flavonoids in the flower of Salix caprea L. Methods Using rutin as the reference substance, the relative correction factors of luteolin-7-O-β-D-pyranoside, apigenin-7-O-β-D-pyranoside, luteolin and apigenin were calculated by high performance liquid chromatography(HPLC). Related influencing factors were investigated and the relative correction factors were determined. The content of the other four components was calculated directly using the content of rutin and the relative correction factors to establish the QAMS method with rutin as the reference substance. The results were compared with those obtained by external standard method. Results The relative correction factors were fluteolin-7-O-β-D-pyranoside/rutin =1.49(RSD=1.84%), fapigenin-7-O-β-glucopyranoside/rutin =2.26(RSD=2.26%), fluteolin/rutin =2.63(RSD=1.01%) and fapigenin/rutin =2.86(RSD=1.55%), with good repeatability. There were no significant differences between the values obtained with the QAMS method and those obtained with external standard method, and the correction factor obtained was reliable. Conclusion It is feasible and accurate to determine the content of five flavonoids in flower of Salix caprea L. by the QAMS method, which can be used for quality evaluation of the flower.
作者
刘倩芸
李新霞
迪娜.吐尔洪
许芳
李建光
LIU Qianyun;LI Xinxia;Dina TUERHONG;XU Fang;LI Jianguang(College of Pharmacy,Xinjiang Medcial University,Urumqi 830011,China)
出处
《上海中医药杂志》
2019年第3期100-104,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
新疆维吾尔自治区重点研发项目(2016B03044-4)
