摘要
目的观察大蒜素对大鼠肝纤维化的防治效果,并探讨其机制。方法将45只大鼠随机分为6组,其中模型组、小剂量预防组、中剂量预防组及中剂量治疗组均采用四氯化碳(CCl4)腹腔注射的方法建立大鼠肝纤维化模型,小、中剂量预防组于造模开始分别给予大蒜素11、22 mg/(kg·d)灌胃,中剂量治疗组则于造模后第5周给予大蒜素22 mg/(kg·d)灌胃;空白对照组不做处理,溶剂对照组给予等量植物油灌胃及腹腔注射,共8周。采用ELISA法检测各组血清ALT、AST、生长转化因子β(TGF-β),光镜下观察肝细胞变性、胶原纤维增生程度并进行评分。结果各组血清ALT、AST比较,模型组>小剂量预防组>中剂量预防组、中剂量治疗组、空白对照组、溶剂对照组,P均<0.05。各组血清TGF-β比较,模型组>小剂量预防组>中剂量预防组>中剂量治疗组>空白对照组、溶剂对照组,P均<0.05。病理组织学检查结果显示,小、中剂量预防组及中剂量治疗组肝细胞变性和纤维化程度明显减轻,肝组织结构比较完好。各组肝细胞变性程度及胶原纤维增生程度评分比较,模型组>小剂量预防组、中剂量预防组、中剂量治疗组>空白对照组、溶剂对照组,P均<0.01。结论中等剂量的大蒜素可较好地防治大鼠肝纤维化,可能与其降低TGF-β表达及减少肝细胞变性有关。
Objective To observe the protective effect of allicin on hepatic fibrosis in rats,and to investigate its mechanism. Methods Forty-five Wistar rats were randomly divided into six groups as follows: blank control group,solvent control group,model group,low-dose allicin prevention group,medium-dose allicin prevention group and mediumdose allicin treatment group. Rat models of hepatic fibrosis in the model,low-dose prevention allicin,medium-dose prevention allicin and medium-dose allicin treatment groups were induced by carbon tetrachloride( CCl4) intraperitoneally for eight weeks. Rats in the low-dose and medium-dose allicin prevention groups received allicin( 11,22 mg /( kg·d)) at the beginning of modeling. Rats in the medium-dose allicin treatment group received allicin( 22 mg /( kg·d)) from the fifth week. Rats in the solvent control group was treated with the same amount of vegetable oil for 8 weeks. The expression levels of ALT,AST and transforming growth factor-β( TGF-β) were detected by ELISA. The hepatic histological changes were also observed by optical microscopy as well as the hyperplasia degree of collagen fiber. Results Comparison of ALT,AST serum levels: model group > low-dose allicin prevention group > medium-dose allicin prevention group,medium-dose allicin treatment group,blank control group,or solvent control group( all P < 0. 05). Comparison of TGF-β serum levels:model group > low-dose allicin prevention group > medium-dose allicin prevention group > medium-dose allicin treatment group > blank control group or solvent control group( all P < 0. 05). The histopathological results showed that allicin could attenuate the degree of hepatic fibrosis and cellular degeneration and protect the entire structure of liver tissues. Comparison of liver cell degeneration degree and collagen fiber hyperplasia degree evaluation: model group > low-dose allicin prevention group,medium-dose allicin prevention group,medium-dose allicin treatment group > blank control group,solvent control group( all P < 0. 01). C
出处
《山东医药》
CAS
北大核心
2015年第16期5-7,111,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81360507
81060274)
关键词
大蒜素
肝纤维化
生长转化因子β
大鼠
allicin
liver fibrosis
transforming growth factor-β
rats
