摘要
目的观察大蒜素对大鼠肝星状细胞(HSC-T6)增殖及细胞外基质降解酶-金属蛋白酶抑制剂-1(TIMP-1)的影响,探讨大蒜素抗肝纤维化的作用及其机制。方法体外培养大鼠肝星状细胞株HSC-T6。MTT法检测大蒜素作用下对大鼠肝星状细胞增殖的影响、Real-time RT-PCR检测肝星状细胞的TIMP-1mRNA水平、Western blot检测肝星状细胞的TIMP-1蛋白的表达水平。结果 HSC-T6大蒜素处理组与空白对照组比较,增殖抑制率明显提高,差异有统计学意义(P<0.01);TIMP-1的mRNA及蛋白表达水平均明显下降,并且大蒜素浓度增高时,TIMP-1的mRNA及蛋白表达水平则逐渐下降,大蒜素高浓度(24μg/mL)组对TIMP-1的抑制作用明显高于空白对照组,差异有统计学意义(P<0.05)。结论大蒜素对HSC-T6增殖和TIMP-1的表达均有抑制作用,为临床开发抗肝纤维化的新药物提供了理论基础。
Objective To observe the effect of allicin on rat hepatic stellate ceils （HSC-T6） proliferation and tissue inhibitor of metalloproteinase-1 （TIMP-1） expression, and its antifibrotic activity. Methods HSC-T6 cells were cultured in vitro. MTT assay was used to measure the proliferation of HSC-T6 cells. Real-time PCR and western blot were used to detect the expression of TIMP-1 mRNA and protein. Results Compared with the control group, the proliferation of HSC-T6 cells was inhibited when treated with allicin （P 〈 0.01 ）. Meantime, the expression of TIMP-1 mRNA and protein was significantly decreased in alliein-treatment group, especially when the concentration of allicin was 24 μg/mL（ P 〈 0.05 ）. Conclusion Allicin plays an important role in inhibition of HSC-T6 cells proliferation and TIMP-1 expression, which may provide an evidence for anti-hepatic fibrosis drugs research.
出处
《广东药学院学报》
CAS
2013年第6期656-659,共4页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学基金(81060274)
石河子大学科学技术研究发展计划项目(RCZX201025)
关键词
大蒜素
肝星状细胞
细胞增殖
金属蛋白酶抑制剂-1
allicin
hepatic stellate cells
cell proliferation
tissue inhibitor of metalloproteinase-1
