摘要
目的观察大蒜素对大鼠肝星状细胞(HSC-T6)增殖的影响,探讨大蒜素抗肝纤维化的作用及其机制。方法体外培养大鼠肝星状细胞株HSC-T6。以四甲基偶氮唑盐(MTT)法测不同浓度大蒜素作用对大鼠肝星状细胞的影响;以碘化丙啶染色流式细胞术(PI-FCM)检测大蒜素对大鼠肝星状细胞的抑制作用。结果 HSC-T6大蒜素各浓度处理组与空白对照组及溶剂对照组比较,增殖抑制率明显增高,有显著性差异(P<0.01);并且大蒜素浓度增高时,HSC-T6的抑制率也逐渐增强;DATS高浓度实验组(浓度含量12μg/ml DATS)的增殖抑制率明显高于空白对照组、试剂对照组及低浓度实验组。结论大蒜素可以抑制HSC-T6增殖,且作用具有剂量依赖性。大蒜素抑制HSCT6增殖的机制可能是使HSC-T6细胞周期停滞。
Objective To observe the allicin rat hepatic stellate cells （ HSC - T6 ） proliferation, and to explore allicin anti - liver fibrosis and its mechanism. Methods In vitro culture of rat hepatic stellate cell line HSC T6, different concentration of allicin MTI＂ assay at 12 h, 24 h or 48 h in rat hepatic stellate cells were analyzed and compared. The inhibitory effect were detected by PI - FCM on rat hepatic stellate cell. Re- suits HSC - T6 large allicin DATS were compared at each concentration treatment group, blank control group and solvent control group. The pro- liferation inhibition rate was significantly higher, and there are significant difference （ P 〈0.01 ）. DATS concentration increased, the HSC -T6 the inhibition rate also gradually enhanced. Proliferation inhibition rate of the experimental group were exposed to DATS. The high concentrations （ concentration content 12 μg/ml DATS） was significantly higher than those of the control group, the reagent control group and the low concentra- tion of the experimental group. Conclusion Allicin can inhibit the proliferation of HSC - T6 role in a dose - dependent manner. Allicin inhibits the proliferation of HSC - T6 mechanism may HSC - T6 cell cycle arrest.
出处
《临床和实验医学杂志》
2014年第3期161-165,共5页
Journal of Clinical and Experimental Medicine
基金
国家自然科学基金<大蒜素对大鼠肝星状细胞胶原生成的调节作用及其相关差异表达基因靶点的筛选研究>
基金编号:81060274
石河子大学科学技术研究发展计划项目<大蒜素抗肝纤维化的多效性机制:细胞增殖动力学和蛋白质组学研究>
基金编号:RCZX201025
关键词
大鼠
肝星状细胞
细胞增殖
大蒜素
细胞凋亡
Rat
Hepatic stellate cells
Cell proliferation Allicin
Cell cycle
